55 



MARVIN ALLUM 



closures. Samples were air transported to the Washington Stat^ 



Department of Fisheries Shellfish Laboratory for the bioassays 



In the waste sample study 24-hour composite 

 samples were collected from the individual mill waste streams. 

 Aliquots of these samples were tested for sulfite waste 

 liquor and other components and the samples were shipped 

 under refrigeration again to the fisheries laboratory at 

 Point Whitney, where they were immediately prepared for 

 bioassay. Serial dilutions of one part of waste sample 

 to lOj 20, 100, 200, and so on, parts of seawater from the 

 laboratory supply were made. For each waste sample, percent 

 abnormal values from the several dilutions were plotted on 

 probit paper against the appropriate dilution ratios and 

 sulfite waste liquor concentrations. From the line of 

 best-fit, dilution ratios and associated sulfite waste 

 liquor values for the 0, 20, 50 and 100 percent abnormal 

 levels were determined. 



All bioassays and associated laboratory 

 analyses were performed or supervised by Charles Woelke 

 of the laboratory's staff. The results of the waste 

 sample study were analyzed and evaluated by Mr. VJoelke; 

 the results of the field sample response study were 

 statistically analyzed by Dr. Gerald Paullk, who is a 

 Biometrician at the Washington University School of Fishcr.ies. 



