62 



1 MARVIN ALLUM 



2 (Slide FG-21) 



3 Then to each of the three beakers for each 



4 sample an aliquot portion of eggs is distributed to try 



6 to get from 20,000 to 30,000 eggs per beaker. 

 g (Slide FG-22) 



7 The beakers then are incubated at 20 degrees 

 g Centigrade in a water bath. Now, in actual practice these 

 9 beakers would be covered and would be in a somewhat larger 



10 container. 



11 (Slide FG-23) 



12 A8 hours later the eggs are concentrated or 



13 removed from the sample by pouring the sample through a 



14 35 -micron mesh sieve. Note that the water passing 



15 through is being collected. 



16 (Slide FG-24) 



17 The water minus the eggs is again subjected 



18 to the Pearl-Benson Index for sulfite waste liquor to 

 10 insure that no changes have occurred during the 48-hour 



20 period and the salinity is also checked to insure that 



21 this parameter has not changed during the test. 



22 (Slide FG-25) 



23 The eggs then that are collected in the 



24 35-micron screen collector are subsequently washed into 



25 a beaker of sterile filtered seawater. 



