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JOHN VLASTELICIA 

 a cessation in pulp oilll operations, such as during the 

 labor strike. 



FLATFISH STUDIES 



Now, the Project also conducted similar 

 bioassay studies using the eggs of English sole, a com- 

 mercially important flatfish of Puget Sound. The English 

 sole, along with many other important species of flatfish, 

 and other fishes as well, hake, codfish, and so on, spawn 

 in the shallower bays of Puget Sound. The eggs of these 

 fishes float after hatching and subsequent embryonic and 

 larval development takes place in the near-surface waters. 

 Flatfish egg occurrence studies demonstrated that sub- 

 stantial numbers of these eggs are found in the zones of 

 chronic pollution in both the Bellingham and Everett study 

 areas. 



The English sole egg bioassay study was 

 conducted at the University of Washington's Friday Harbor 

 laboratory in a manner very similar to the waste sample 

 portion of the oyster larva bioassays. English sole eggs 

 were fertilized in tbe laboratory and incubated in dilutions 

 of sulfite waste liquor ranging in concentrations from 

 6 parts per million to 1,000 parts per million. After 

 incubation, the eggs and larvae were examined under a 



microscope to determine the response, either in terms of 



