APPENDIX F. SENTINEL ANIMAL PROGRAM 



Methods 



Rodents used in the Carcinogenesis Program of the National Toxicology Program are produced in op- 

 timally clean facilities to eliminate potential pathogens that may affect study results. The Sentinel 

 Animal Program is part of the periodic monitoring of animal health that occurs during the toxicologic 

 evaluation of chemical compounds. Under this program, the disease state of the rodents is monitored 

 via viral serology on sera from extra (sentinel) animals in the study rooms. These animals are un- 

 treated, and these animals and the study animals are both subject to identical environmental condi- 

 tions. The sentinel animals come from the same production source and weanling groups as the ani- 

 mals used for the studies of chemical compounds. 



Fifteen B6C3Fi mice and 15 F344/N rats of each sex were selected at the time of randomization and 

 allocation of the animals to the various study groups. Five animals of each designated sentinel group 

 were killed at 6, 12, and 18 months on study. Data from animals surviving 24 months were collected 

 from 5/50 randomly selected control animals of each sex and species. The blood from each animal was 

 collected and clotted, and the serum was separated. The serum was cooled on ice and shipped to 

 Microbiological Associates' Comprehensive Animal Diagnostic Service for determination of the viral 

 antibody titers. The following tests were performed: 



Hemagglutination Complement 



Inhibition Fixation ELISA 



Mice PVM (pneumonia virus of mice) M. Ad. (mouse adenovirus) MHV (mouse hepatitis 



Reo3(reovirus type 3) LCM (lymphocytic chorio- virus) (12, 18, 24 mo) 



GDVII (Theiler's encephalo- meningitis virus) 



myelitis virus) MHV (6 mo) 



Poly (polyoma virus) Sendai(18mo) 



MVM (minute virus of mice) 

 Ectro (infectious ectromelia) 

 Sendai(6, 12,24mo) 



Rats PVM RCV(ratcoronavirus) 



KRV(Kilham rat virus) SendaidSmo) 



H-KToolan'sH-l virus) 

 Sendai (6, 12, 24 mo) 



Results 



Results are presented in Table Fl. 



Nitrofurantoin, NTP TR 341 202 



