TABLE 5. EXPERIMENTAL DESIGN AND MATERIALS AND METHODS IN THE FEED STUDIES OF 



NITROFURANTOIN (Continued) 



Fourteen-Day Studies 



Thirteen-Week Studies 



Two-Year Studies 



ANIMALS AND ANIMAL MAINTENANCE (Continued) 



Time Held Before Study 



Rats- 21 d;mice -14d 



18d 



16d 



Age Wlien Placed on Study 

 Rals--7-8 wlc; mice-6-8 wlc 



Rats-8 wk; mice-8-9 wk 



Rats 6-7 wk; mice -8-9 wk 



Age When Killed 



Rats--9- 10 wk; mice-8-10 wk 



Rats-22-23 wk; mice-22-24 wk 



RaU -110- 1 12 wk; mice -112-114 wk 



Necropsy Dates 



Rals--3/5/80-3/7/80; 

 mice--3/27/80-3/28/80 



Rats-8/6/80-8/15/80; 

 mice-8/4/80-8/14/80 



Rats -2/25/83-3/2/83; 

 mice-2/18/83-2/23/83 



Method of Animal Distribution 



Animals distributed to weight 

 classes and assigned to cages accord- 

 ing to one table of random numbers; 

 cages assigned to groups according to 

 another table of random numbers 



Same as 14-d studies 



Same as 14-d studies 



Feed 



NIH 07 Rat and Mouse Ration 

 (Zeigler Bros.. Inc., Gardners, PA); 

 available ad libitum 



Same as 14-d studies 



Same as 14-d studies 



Bedding 



Beta Chip® hardwood chips 

 (Northeastern Products Corp., 

 Warrensburg, NY) 



Same as 14-d studies 



Same as 14-d studies 



Water 



Automatic watering system 

 (Edstrom Industries, Waterford, 

 Wl ); available ad libitum 



Same as 14d studies 



Same as 14-d studies 



Cages 



Polycarbonate (Lab Products, 



Inc., Garfield, NJ) 



Same as 1 4-d studies 



Same as 14-d studies 



Cage Filters 



Reemay spun-bonded polyester 

 fillers (Snow Filtration, 

 Cincinnati, OH) 



Same as 1 4-d studies 



Same as 14-d studies 



Animals per Cage 



5 



Other Chemicals on Study in the Same Room 



None None 



Animal Room Environment 



Temp -22°-23°C;hum-41%-52% 

 (rats), 41%-49% (mice); fluorescent 

 light 12 h/d; 15 room air changes/h 



Temp-22°-24° C; hum--44%-70%; 

 fluorescent light 12 h/d; 15 room 

 air changes/h 



None 



Temp-17.2°-31.1°C;hum-50% ± 10%; 

 fluorescent light 12 h/d; 15 room air 

 changes/h 



31 



Nitrofurantoin, NTP TR 341 



