COAL-CONVERSION GASIFIER CONDENSATE 



209 



60 120 180 240 



LENGTH OF EXPOSURE, min 



300 



Fig. 1 Effect of gasifier condensate on respiration of Tetrahymena 

 at 28°C. 



immediate 5- to 7-unit decrease in the percent-saturation reading 

 (Fig. 2, curve A). The total time elapsed from the addition of 

 toxicant to reestabhshment of a linear decrease in percent saturation 

 (which we refer to as reaction time) was ~30 sec. In this case 

 introducing half-strength condensate caused an immediate 1-unit 

 decrease in the percent saturation (Fig. 2, curve B); the reaction time 

 was less than 15 sec. The concentration of test solution added 

 affected the slope (rate of respiration) of the reestablished oxygen- 

 consumption line, as well as the reaction time and percent saturation. 

 Adding 0.3 ml of sterile medium to the cell suspension caused a very 

 slight decrease in the rate of oxygen uptake, less than 0.5%/ min 

 (Fig. 2, curve C). Sterile medium injected into sterile medium had no 

 effect (Fig. 2, curve F). FuU-strength condensate introduced into 

 sterile medium caused a decrease in saturation of about 6 to 8 units, 

 udth a reaction time of less than 15 sec (Fig. 2, curve D). Half- 

 strength condensate added to sterile medium caused only a 3% 

 decrease wdthin 9 sec (Fig. 2, curve E). These tests showed that, 

 Eilthough the addition of gasifier condensate initially reduced the 

 oxygen content of the medium, respiration was £ilso reduced. 



