REPRODUCTION IN SEA ANEMONE 



471 



SCALE 



o POWER PLANT 

 o 

 o 



NTAKE 



MORRO BAY 



Fig. 1 Collection sites near a 1350-MW Pacific Gas and Electric 

 Company power plant at Morro Bay, San Luis Obispo County, 

 California. 



summer, and spawning occurring in the early fall. He speculated that 

 reproduction might somehow be cued by the increasing seawater 

 temperature in summer. 



My purpose in this study was to determine whether the timing of 

 reproductive events in this species was altered by subjection to the 

 elevated temperature of a power-plant thermal-outfall canal. 



MATERIALS AIMD METHODS 



Twelve to 24 anemones were collected at both control and 

 outfall sites (Fig. 1) at 4- to 8-week intervals between Feb. 4, 1974, 

 and Sept. 27, 1976. Animals were relaxed in 50 : 50 seawater and 

 isotonic MgClj, cleaned, fixed in Bouin's fluid, dehydrated, em- 

 bedded in paraffin, and sectioned at thicknesses of 8 to 10 jim on a 



