88 



Fishery Bulletin 93|1). 1995 



Sphyraena sphyraena 

 Coryphaena equiselis 

 Morone saxatilis 

 Mycteroperca interstitialis 

 Crossostoma lacustre 

 Cyprinus carpio 



Figure 4 



Phytogeny of the Scombroidei based on a weighted, maximum parsimony analysis of informative 

 nucleotide sites. The six types of nucleotide substitutions are weighted according to the ratio of 

 their expected occurrence to their observed occurrence (see Table 3). Weights used for each sub- 

 stitution type are the following: A«=>G=1, CoT=l, Gt=>T=13, GoC=4, A<=>T=2, and AoC=2. 

 Crossostoma and Carpio were specified as the outgroup. The tree depicted is the single most 

 parsimonious topology identified in a heuristic search: TBR branch swapping was performed on 

 10 starting trees generated through random stepwise addition of taxa. Tree length is 2,348 steps. 

 PAUP 3.1. was unable to derive consistency and retention indices for the cladogram that incorpo- 

 rated the weighting scheme. Circled numbers at nodes indicate the percentage of trials in which 

 a given partition between taxa is supported in 1,000 replications of the bootstrap analysis 

 (Felsenstein, 1985). 



This fragment spans four transmembrane domains 

 and includes part of the region implicated as the outer 

 membrane redox reaction center (Howell and Gilbert, 

 1988; Howell, 1989; Fig. 6). In a comparison of the 



inferred peptide sequences across the 36 species in- 

 cluded in this study, 134 (69%) of the 195 amino acid 

 residues are invariant, 34 (17%) occur in 2 amino 

 acid states, and 27 (14%) occur in 3 or more states. 



