86 



Fishery Bulletin 93(1), 1995 



440 460 480 500 520 540 560 580 



1 CTGCTATGACTCTAATCCACCTCCTrTTCClOCACGAAACAaG^ 



2 C. .A C Ft. .A A. .G G T C 



.C. .R. 

 .C. .A. 



.A 



.GC... 

 .G..G. 



A. 



.G..A. 



.C. .A. 

 .C. .A. 



.GC... 

 .G. .G. 

 .G..G. 



.G. .C. 

 .G. .C. 



9 TA..CGCA. .CA 



10 .A..C. .A. .AA 



11 TC A. .AA 



12 TG..C. .A. .AA 



13 TG..C. .A. .AA 



14 .A A 



15 .A A. .AA 



16 -A A. .AA 



17 .A..C AA 



18 .A. .C AA 



19 .A.. 



20 .A.. 



21 .A.. 



22 .A.. 



23 TA. . 



24 TA..C..A..AA 



25 TG..AGCA..AA 



26 TA. .GGCAG.G. 



27 .A. .A AA 



28 .A AT. A. 



29 TG. .A. .A. .AA 



30 TA..03CT..A. 



31 T. .G.G.C.CC 



32 TG..A.CT...T 



33 GCC. .C. 



34 .A..CT.T. .AC 



T. .T. 

 TC. .. 



• A 



.A. .A. 



TC.T A. .G..T..G. 



.C. .C. .C A. .CC.C. .T C C. 



.T. .C A.TC. .AY TA.T C. 



.C T A.TA. .AC A.T C 



G. 



.T..A. 



.C.C 

 .C. 



.C T C. 



.C A.T... A. 



.C AAT. . .A. 



TC.T A A T T. .T T. . .AC. .GT. . .TA C C 



TC.G. .T. .A. .C. .T. .T. .T C A.TC. .CC.C A C C 



TC A A G. .C. .A. .T. .T T. . .A A.T. .C.C C. .T. 



..C C.C. AAT. 



.AC C.C.T.T. 



..CT. .C.C. AAT. 



OCT A A C. .A A.TC. GC.G. .T. . .A.T C C .T A. .T T C.C. .C .C. .AAT. T.A 



TC.T A A C .? A.T. . .AC A C C .C A C.T. .C AAT. T.A 



TC A T C. .A Y. .Y. .A.T. . .A A.T. .G. .C C .C A C.T. .C. .C. .AATT. A 



TC.T. 



T T T. .T. .A.TC. .A A.T C 



.C.T. 



.C.TGAT. 



. . .AA.TC.T T T T T. .A.TC. -A 



. . .AA 

 . . .AA 

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TC.T. . . . 

 TC.T. .T. 

 TC.T. . . . 



TC.T 



TC.T. . . . 

 TC.T. . . . 

 TC 



T 



T 



A.. 



.G..A.. 

 .A. .A.. 



. .A. .T C. . 



. .T. .T T A.T. . .CC TA.T C. 



. X. .C. 

 . .C..G. . 



T.. 



T.. 



A.. 



.T. -G. . 



...C.T C.TGAT... G 



. ..C.T C.TGAT.. .G 



...C.T C.TGAT... G 



. ..C.T C.TGAT... G 



. .TC.T. .A. .C. .AATT. .G 



..TC.T. .A AATT. .G 



...C.T..C T. ..C 



TA.G. .T C.C .C TT. .C. .T. .A T 



T... 



. .T..A..T 



A..T 



. . .A 



.03... CA. 



CA. 



. -CCTCA. 

 .CCCACG. 

 .GG.G.CA. 

 . .G.G.CA. 

 . .G.A.CA. 

 . .G.A.CA. 

 . .G.A.CT. 

 . .G.G.CA. 

 ..G.A.CA. 

 . .G.A.CG. 

 . .G.A.CA. 

 . .G.A.CA. 

 .TO.. .CA. 

 .TG...CA. 

 .CG.A.C. 

 T. .A. 



-CG 



.03T 



.CGT.A. . 



.TG 



. .G.T.. . 



.T3 



.TO 



.CG.T. .T 



03 



CG 

 .CG 

 .03 

 .03 

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..T 

 ..T 

 ..T 

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 ??? 

 ..T 



TC T..A. .C T..T..G..C T T C.C A C C. .C T C GAT. . .AG.G C ..GG..??? 



GC.A A A A. .T A.T. . .CC.C A C A TC A. ,C .T.TA. .A A.C G 



CC.T G T T A.T. . .AC.C TA C C T. .G T CG. .C AAT. . .C. . .G. . -CA. -T T 



.C.T. .T C G. .C C.T GCTA. .ACT A. . -T. . .C C T. -T. .T. .T T. . .C.T. .A AATTA.A. .C. .OCT. . .C . .A.T 



CG.T AA.C A G A. .C ACT3. .ACT A. . .T. . .C .G. .C A. A. A CG. .C TA. .G. .C. .C.C .C. . .C. .TAG. 



.G. . . -T. -A. .A T G. .T. .C .C .T T CC T. . .T.C OC.C A T. .T TC.ACT. .C . .A. A.T. . .GT.G3. ,CTT. .C. -A. . . 



CT.A TT.G T C TEA. .OCT T. .T.T. . .C CC T T G T G. -C. -A. . -G.C . .G G T 



TT T T GTT. . .C CT3. .GC.T T C.C A T. .A. .T T. .TC A A G. .C .CCA. -C .A. . . 



Figure 3 (continued) 



the inferred number of substitutions (Table 3). For 

 example, if a nucleotide site is twofold variable, i.e. 

 if two bases occur at that position in an alignment of 

 all species, then at least one base substitution has 

 occurred at that position during the evolutionary his- 

 tory of the species concerned. Likewise, if a position 

 is threefold variable, at least two substitutions have 

 occurred, and so on. By this approximation, the 293 

 variable positions have experienced at least 521 sub- 

 stitutions, and substitutions at the third position 



outnumber substitutions at the first and second po- 

 sitions by nearly 4 to 1 and by more than 12 to 1, 

 respectively. 



Figure 2 presents a phylogeny of the Scombroidei 

 based on an unweighted parsimony analysis of all 

 informative nucleotide sites. In this cladogram, only 

 the relationships among recently diverged taxa are 

 strongly supported. There is support for the mono- 

 phyly of genera within the family Scombridae 

 (Thunnus, Euthynnus, Sarda, Scomber, and Scorn- 



