186 BULLETIN OF THE UNITED STATES FISH COMMISSION. 



eggs, and required continual watchfulness on the part of the attendant 

 to prevent considerable losses of live eggs. A second form of appa- 

 ratus, looking to the accomplishment of the same result, is shown in 



Fig. 2. 



The results with these forms of apparatus were not satisfactory in 



developing a method which could be conveniently applied in practice, 

 yet they pointed the way to it. Later in the spring, near the close of 

 the hatching season, at the suggestion of Professor Baird, and in con- 

 junction with Professor Ryder, we instituted, in the basement of the 

 Smithsonian Institution, a series of experiments in order to determine 

 the limit of healthy retardation of development that could be effected 

 by lowering the temperature of the water employed. In order to sub- 

 ject the eggs conveniently to the action of the current of cold water 



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Fig. 3.— Original form of apparatus in which the method for automatic separation of dead from liv- 

 ing eggs was demonstrated. 



they were placed in small two-ounce laboratory flasks, closely corked. 

 Through the center of the cork was passed a glass tube which descended 

 to within a short distance of the bottom of the flask, and through which 

 the current of water was admitted to the apparatus. This is shown in 

 Fig. 3. 



