FISHERY BULLETIN: VOL. 85, NO. 4 



(Table 2). Initially all 30 enzymes were examined 

 in all fish; in later samples, however, we omitted 

 the loci in chinook salmon that had been deter- 



mined to be monomorphic in previous studies or 

 in our initial screening. 



Table 2. — Enzymes and loci examined in chinook and coho salmon. Enzyme commission numbers are in parentheses. Tissue 

 E refers to eye, H to heart, L to liver, and M to white muscle. Buffer system 1 was described by Ridgway et al. (1 970), 2 by Clayton 

 and Tretiak (1972), and 3 by Markert and Faulhaber (1965) and Kobayashi et al. (1984). 



Enzyme 



p-N-Acetyl-galactosaminidase (3.2.1 .23) 



N-Acetyl-B-glucosaminidase (3.2. 1 .30) 

 Acid phosphatase (3.1.3.2) 



Aconitate hydratase (4.2.1.3) 



Adenosine deaminase (3.5.4.4) 



Adenylate kinase (2.7.4.3) 

 Alanine aminotransferase (2.6.1.2) 

 Alcohol dehydrogenase (1.1.1.1) 

 Aspartate aminotransferase (2.6.1.1) 



Creatine kinase (2.7.3.2) 



Diaphorase-NADH (1.6.*.*) 



Diaphorase-NADPH (1.6.*.*) 



Fructose bisphosphate aldolase (4.1.2.3) 



Fumarate hydratase (4.2.1.2) 

 Glucose-6-phosphate isomerase (5.3.1.9) 



p-Glucuronidase (3.2.1.31) 

 Glyceraldehyde-3-phosphate dehydrogenase (1.2.1.12) 



Glycerol-3-phosphate dehydrogenase (1.1.1.8) 



L-lditol dehydrogenase (1.1.1.14) 

 Isocitrate dehydrogenase (1.1.1.42) 



L-Lactate dehydrogenase (1.1.1.27) 



Lactoylglutathione lyase (4.4.1.5) 

 fylalate dehydrogenase (1.1.1.37) 



Malate dehydrogenase (NADP + ) (1.1.1.40) 



IVIannose-6-phosphate isomerase (5.3.1.8) 

 a-Mannosidase (3.2.1.24) 

 Phosphoglucomutase (5.4.2.2) 



Phosphogluconate dehydrogenase (1.1.1.44) 

 Phosphoglycerate kinase (2.7.2.3) 



Superdioxide dismutase (1.15.1.1) 



^ No Isozyme variation observed. 



684 



