FISHERY BULLETIN: VOL. 85, NO. 4 



Table 3.— Chinook salmon — coefficients for contrasts (Snedecor and Cochran 1967) 

 within the analysis of variance. Cell numbers refer to groups identified in text. Within 

 each contrast, the mean allele frequencies for groups with positive coefficients were 

 compared with the mean frequencies for groups with negative coefficients. 



salmon from the Quinault River were not in- 

 cluded because many hatchery fall chinook 

 salmon return to the Quinault River with the 

 summer-run salmon (during August, when most 

 of our sampling was done) and our samples prob- 

 ably included a high proportion of fall-run hatch- 

 ery fish (Larry Gilbertson'*). 



Gene Diversity Analysis 



We used a modification of Chakraborty's (1980) 

 gene diversity analysis to examine the hierarchi- 



4 Larry Gilbertson, Quinault Tribal biologist, Quinault In- 

 dian Nation, P.O. Box 189, Taholah, WA 98587, pers. commun. 

 August 1983. 



cal structure of genie diversity among the sam- 

 ples of wild coho salmon from the north coast. 

 This analysis partitions total gene diversity (//,, 

 heterozygosity of allele frequencies over loca- 

 tions) into interdrainage and intradrainage com- 

 ponents (Nei 1973). We considered three levels of 

 population subdivision (Fig. 2) — broods (6), 

 streams within drainages (w), and drainages 

 id) — so that Hi = Hg + Di,w + 0^,^ + D^t, where 

 Hg is the average heterozygosity within samples, 

 Z)^y, is the gene diversity between broods, 0^,^ is 

 the diversity within drainages, and Z)^/ is the di- 

 versity among drainages. Relative gene diversi- 

 ties {G,j ) are the proportions of //^ associated with 

 a particular hierarchical level; for example, 

 Gwd = Du,d/Ht. 



Drainage 



Quillayute River 



North Coast 

 Coho Salmon 



Hoh River 



Queets River 



Stream 



Dickey River 



Soleduck River 



Quinault River 



.Bogachiel River 



vCalawah River 



Clearwater River 



Brood 



1981 



< 



1982 



1982 



1982 



1981 



< 



1982 



■' ^Upper Queets River 1982 



1982 



Figure 2. — Coho salmon — hierarchical subdivisions used in the gene diversity analysis for wild fish 

 from the north coast of Washington (see test). Where brood is not identified, fish were from both the 

 1981 and 1982 broods. 



686 



