FISHERY BULLETIN: VOL. 85, NO. 4 



most similar to wild fish. Summer-run adults and 

 fall-run adults from the Quillayute River both 

 clustered with the wild fish, suggesting that a 

 large proportion of the fish in these samples were 

 wild fish. Multidimensional scaling gave similar 

 results and more clearly illustrated that hatchery 

 populations were distinct not only from the wild 

 fish but also from each other (Fig. 5). 



Coho Salmon 



Coho salmon showed genie variability at 21 loci 

 or pairs of loci; however, the frequency of the 

 common allele was <0.95 for most samples at 

 only 2 loci: bGala-2 and Idh-3,4 (Fig. 6, App. 

 Table 2). Allendorf and Utter (1979) found a sim- 

 ilar lack of variation, reporting that coho salmon 

 display the least amount of electrophoretic varia- 

 tion of the five Pacific salmon species in North 

 America. 



Hierarchical analysis of genie diversity (het- 

 erozygosity) showed that the interbrood level ac- 

 counted for 2% (= 0.09/(0.09 + 0.85 + 3.97; Table 



6) of the genie diversity observed among samples 

 of coho salmon; the within-drainage level ac- 

 counted for 17% and the interdrainage level for 

 81%. Variation at Pnp-1 had a substantial influ- 

 ence on the average locus values. Unfortunately, 

 data for Pnp-1 were missing for several of the 

 samples because the methodology for this enzyme 

 was not stabilized until we were well into our 

 study. With Pnp-1 excluded from the analysis, 

 the interbrood level accounted for 5% of the genie 

 diversity observed among samples, the within- 

 drainage level accounted for 39%, and the in- 

 terdrainage level accounted for 56%. 



Variation in allele frequencies among streams 

 within the Quillayute and Queets drainages was 

 statistically significant (tested at bGala-2, Idh- 

 3,4, and Pnp-1 ; G = 11.27 with 5 degrees of free- 

 dom; P < 0.05); however, interpretation of this 

 result is complicated because data were not avail- 

 able to adequately account for variation among 

 year classes. Variation among drainages was not 

 significantly greater than variation within 

 drainages (P > 0.10, hierarchical likelihood ratio 



S OLEOUCK H.--FR 



o 



SOLEDUCK H.-- SR 



QUINAULT PENS 



Q 



QUINAULT ADULTS 



ELWHA HATCHERY 



OUINALT NFH 



Figure 5. — Chinook salmon — two-dimensional representation (from multidimensional scaling) of ge- 

 netic distances among samples collected for this study. The letters correspond to the groups identified 

 in Figure 4. The polygon encloses the samples of wild fish (A through N). The aim of multidimensional 

 scaling is to represent each group by a point in two-dimensional space so that the relative distances 

 among points represent the relative (genetic) distances between groups. 



690 



