Lobster Hepatopancrcas, and TORT-1 Lobster Hepatopancreas. NIST Standard 

 Reference Solutions were used for instrument calibration. Five to 29 tissue 

 replicate CRM analyses were done for the various metals. 



Chlorinated Hydrocarbons. Quality assurance measures for CHs included 

 the analyses of method blanks, replicate analyses of a frozen wet tissue standard 

 reference material (NIST SRM 1974) and a duplicate sample. The SRM is 

 certified for selected PAHs by NIST and reported along with non-ccrtified values 

 for selected CHs. Analyte concentrations were reported on the basis of the 

 surrogate standard dibromooctafluorobiphenyl added at the beginning of the 

 sample extraction. Graduated concentrations of GC-calibration-check standards 

 were used for multilevel response-factor determinations. The criteria for 

 instrument stability was that the response for each analyte or surrogate in a GC 

 calibration standard be reproducible within ± 10 %. A method blank and one 

 sample of SRM 1974 were analyzed with each sample set of 10 samples. When 

 the recovery of any surrogate standard for a sample was < 50 %, corrective 

 action was taken, including instrument repair, inlet cleaning, column 

 replacement, and/or reanalysis. 



Analyses for Percent Lipid 



An aliquot of tissue extract was evaporated from a measured Auction of die 

 total tissue extract of each sample to dcf^miine the extractable lipids. Evaluation 

 of the results showed that this procedure gave results for lipids in marine 

 mammal blubber and liver comparable to using the method of Hanson and Olley 

 (1963). 



Analyses for DNA-xenobiotic adducts 



The levels of hepatic DNA-xenobiotic adducts were detemnined by the 



3^P-postlabeling assay modified from Randerath et al. (1984), as described in 

 Varansie/a/. (1989B). 



63 



