fisurej 



(•) 2505-10 fraction 7 [plate 3] 50% di^laoement at 10 unhs or 0.125% of sampie at this stage of puriScation 

 (10 X 0.0125%). 



Therefore, 0.125% * 895 pg eqtiivaients in fraaion 7 from the TLC plate by comparison with standard curves 

 for PbTx-3. 



100%A3.125% - 800, Yielding (895 x 800) « 716,000 pg. or 716 ng in total sample. 



That 716 ng is equivalent to 87.9% of the total sample (100% x 0.958 x 0.958 x 0.958 = 87.9%) 



Therefore, the sample was calculated to contain 815 ng brevetoxin (716 x 100%/87.9%). 



•Based on 45^33 g of liver supplied, approximately 17 ng of brevetoxin per g Bver was present.* 



Figure 4 



(•) CCD94 fraaion 5 [plate 2] 50% displacement at 20 unit or 025% of sample at this stage (20 x 0.0125%). 



Therefore, 025% « 895 pgram equivalents in fraction 5 from the tic plate l^ comparison with standard curves 

 for PbTx-3. 



100%/025% » 400, Yielding (895 x 400)  358,000 pg, or 358 ng in total sample. 



That 358 ng is equivalent to 91 J% of the total sample (100% x 0.958 x 0.958 > 91.8%). 



Therefore, the sample was calculated to contain 390 ng brevetoxin (358 x 100%^1.8%). 



* Based on a weight of 38.036 g liver supplied, approximately 102 ng toxin per g liver.* 



Figure 5 



( •) 2505-5 fraction 3 [plate 2] 50% displacement at 100 units or 125% of sample at this stage (100 x 

 0.0125%). 



Therefore, 1 25% > 895 pg equivalents in fraction 3 from the TLC plate by comparison with PbTx-3 standard. 



100%/125% > 80. Yielding (895 x 80) - 71600 pg. or 71 ng in sample. 



That 71 ng is equivalent to 91 J% of the total sample (100% x 0.958 x 0.958 - 91.8%). 



Therefore, the sample was catailatrd to contain 77 ng brevetoxin (71 x 100%^1.8%). 



'Based on a liver mass of 412S5 g. ap p roxim ately 19 ng brevetaxin ▼« present in the sample.* 



131 



