digestates were diluted with deionized water to a final volume of 25 mL. 

 Selected metals were determined by atomic absoqjtion spectrophotometry: Cold 

 vapor hydride generation was used for determining Hg; acetylene/air flame was 

 used for Fe and Zn; graphite furnace was used for Al, Mn, Ni, Cu, Cr, Sn, and 

 Sb; and Zeeman-corrected graphite fiimace was used for Ag, As, Se, Cd, and Pb. 

 The results for the metal analyses are all discussed on a wet weight basis for this 

 report. 



Analyses for Chlorinated Hydrocarbons 



As with the metals analyses, the analytical methodologies and quality 

 assurance procedures were those used in NOAA's NBSP, with flic procedure for 

 CH analyses modified for the lipid-rich blubber tissue. 



Samples of Aawed liver and blubber tissue from bottlenose dolphins were 

 extracted for CHs modified from procedures of Kiahn et al. (1988). Tissue 

 (1 g) was extracted with methylene chloride following mixing with sodium 

 sulfate, and the mixture was macerated. The extract was filtered through a 

 column of silica gel and alumina, and the extract concentrated for further 

 cleanup. Size exclusion chromatography with HPLC (flow rate of 5 ml/min) was 

 used and a fraction containing the CHs was collected. The dichloromethane 

 solvent in the HPLC fraction was exchanged into hexane as the volume was 

 reduced by evaporation to app^oxi^late^y 1 mL. The extracts were analyzed by 

 capillary column gas chromatography (GC) with an electron capture detector 

 (MacLeod et al. 1985). GC peak identifications were confirmed on selected 

 samples using GC-MS. The results for the CH analyses are aU discussed on a wet 

 weight basis for this report. 



Quality Assurance Measures for Metals and Chlorinated Hydrocarbons 



Metals. Quality assurance included the use of Certified Reference 

 Materials (CRMs), method blanks, solvent blanks, and Certified Calibration 

 Standards. The CRMs used included National Instimte of Standards and 

 Technology (NIST) Standard Reference Materials #1566a Oyster Tissue and 

 #1577a Bovine Liver, and the National Research Council of Canada's (TRM 

 DORM-1 Dogfish Muscle Tissue, DOLT-1 Dogfish Liver, LUTS-1 Non Defatted 



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