TABLE OF CONTENTS 



INTRODUCTION AND BACKGROUND 116 



STATEMENT OF WORK 116 



PRE-AWARD CONDITIONS 116 



AWARD CONDITIONS H*^ 



PROTOCOL FOR LIVER ANALYSES 118 



RATIONALE FOR METHODS 121 



EXPERIENCE, EXPERTISE, AND PROHCENCY OF PERSONNEL 121 



SUBMITTED SAMPLES 122 



FEE SCHEDULE FOR BREVETOXIN ANALYSES 123 



GENERAL OBSERVATIONS ON SAMPLES RECEIVED . 124 



BIOASSAY DURING PURIHCATION 124 



HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 124 



RADIOIMMUNOASSAY 125 



POTENTL\L SOURCES OF ERROR 125 



RECOMMENDATIONS 126 



RADIOIMMUNOASSAY FIGURES AND CALCULATIONS OF 



BREVETOXIN EQUIVALENTS 127 



115 



