COLLINS and TKNNKV: SYSTEM FOR DETERMINING POLLUTANT PARAMETERS 



protein can be calculated from the regression of 

 O&G and protein on TR K . For practical reasons, 

 we prefer using the simultaneous equation be- 

 cause establishing the base data would be difficult 

 at the plant level in that both protein and O&G 

 should be determined and correlated with COD 

 and TR K to establish the accuracy of the analyst. 

 Occasionally, wild values might occur in analy- 

 ses but the average of the standard deviations 

 between duplicate analyses for TR K , FR K , COD TR , 

 and COD FR in this paper was 6.1, 3.6, 14.4, and 

 10.1 mg/liter, respectively. Using the 6 mg/liter 

 TR K figure the predicted value for COD from 

 1,431 ± 12 mg TR K is 1,990 ± 17 mg COD from 

 Equation (1). Based on this interval of two stan- 

 dard deviations, protein and O&G values obtained 

 by the simultaneous equation could vary as 

 follows: 



RECOMMENDATION 



We recommend that this simplified testing- 

 calculating system be used by the fishing industry 

 provided proper regulatory approval is obtained. 

 The following background data will be required: 



1. Determine the regression of COD TR and 

 COD FR on TR K and FR K and calculate the 

 equations [i.e., Equations (1), (2), (3)]. Use 

 grab samples (about 10) to give a good spread 

 of data. 



2. For protein and O&G, either a regression or a 

 simultaneous equation can be used. 



(A) Obtain O&G and protein data on the 

 same samples as above and determine 

 the equation of the regressions of protein 

 and O&G on TR K [i.e., Equations (4) 

 and (5)]. 



(B) Determine the ratio or weight of protein 

 to weight of O&G on several samples 

 and if between 4.6 and 5.9, the constant 

 (1.083) in Equation (8) is assumed 



valid. If not, the constant must be re- 

 calculated in order that the COD TR 

 equals the sum of COD from protein and 

 O&G [see discussion for Equation (6)|. 

 (C) The O&G coefficient should be deter- 

 mined on fishery waste effluents in 

 which the oil may give a significantly 

 different value than 2.678. 



The routine application of this system would be 

 as follows: 



1. Determine COD FR , TR, and ash by direct 

 analysis. 



2. Subtract ash from TR to give TR K . 



3. Substitute into Equations (1) and (2) and solve 

 for COD TR and FR K . 



4. Obtain COD NFR and NFR K by difference or by 

 Equation (3). 



5. Obtain protein and O&G from Equations (4), 

 (5), or (8). 



Thus, three simple and accurate tests give 

 reportable data on nine parameters which more 

 completely describe the pollutant load released to 

 the environment than those currently in use. 



LITERATURE CITED 



Collins, j. 



1976. Oil and grease: A proposed analytical method for 

 fishery waste effluents. Fish. Bull., U.S. 74:681-683. 



Collins, j., and r. d. tenney. 



1976. Fishery waste effluents: A method to determine rela- 

 tionships between chemical oxygen demand and residue. 

 Fish. Bull., U.S. 74:725-731. 

 HORWITZ, W. (editor). 



1965. Official methods of analysis of the Association of Offi- 

 cial Agricultural Chemists. 10th ed. Assoc. Off. Agric. 

 Chem., Wash., D.C., 957 p. 

 JIRKA, A. M., AND M. J. CARTER. 



1975. Micro semi-automated analysis of surface and waste- 

 waters for chemical oxygen demand. Anal. Chem. 47: 

 1397-1402. 



krzeczkowski, r. a., and f. e. Stone. 



1974. Amino acid, fatty acid and proximate composition of 

 snow crab iChionoecetes bairdi). J. Food Sci. 39:386-388. 

 MOORE, W. A., AND W. W. WALKER. 



1956. Determination of low chemical oxygen demands of 

 surface waters by dichromate oxidation. Anal. Chem. 28: 

 164-167. 



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