FISHERY BULLETIN: VOL 75, NO. 1 



TABLE 7. — Release and recovery data on 2,537 rex sole tagged off 

 northern Oregon, April and June 1970. 



Date 



Number Number Percent 

 tagged recovered recovery 



Distance 



traveled 



(km) 



Days at 

 liberty 



April 1970 

 June 1970 



200 

 2,337 





 15 



000 

 0.64 



0.0 



1.5 

 17.1 



0.0 



3.7 

 23.0 

 14.1 



2.2 



8.0 

 14.3 



0.9 



38.9 



539 



unknown 



39 

 523 



0.0 



4 



4 



5 



18 



40 



189 



240 



278 



279 



294 



346 



364 



374 



450 



788 



Total 



2.537 



15 



0.59 



The low returns possibly were caused by rex sole 

 not surviving the tagging process. Manzer (1952) 

 reported rex sole reacted badly to capture and 

 tagging. Most tagged rex sole released at the ocean 

 surface did not immediately descend. Instead, 

 unlike most other flatfish species, they curled into 

 a semicircle and moved across the water surface in 

 a skipping motion. This peculiar reaction might 

 have resulted in a high initial tagging mortality 

 from predation. It may also indicate a stress 

 condition from which fish did not recover. 



Starch-Gel Electrophoretic Analysis 



There were 20 loci detected in the muscle tissue 

 of 145 rex sole. Of these loci 13 were enzymes and 7 

 were muscle proteins (Table 8). Only three of the 

 loci (15%) were polymorphic. 



The polymorphism was found in only three of 

 the eight systems studied or examined. AAT 

 staining occurred in two anodal regions (A-I and 

 A-II). Zone II was the only polymorphic region, 

 having A, B, C, and D alleles (Figure 9, Table 9). 

 The enzyme peptidase also had two anodal re- 



ORIGIN 



ZONE I 

 (POLYMORPHIC) 



ZONE n 

 (MONOMORPHIC) 



AA AB BB BC CC CD DD AD AC BD 

 AAT PHENOTYPES 



FIGURE 9. — Diagrammatic representation of aspartate 

 aminotransferase (AAT) phenotypes in starch gel from 145 rex 

 sole collected off Oregon, April 1973. 



TABLE 9. — Frequencies of aspartate aminotransferase (AAT) 

 phenotypes in 145 rex sole collected off Astoria, Charleston, and 

 Brookings, Oreg., in April 1973. 



gions. Only zone II was polymorphic, with A and B 

 alleles (Figure 10, Table 10). A third enzyme, 

 PGM, was polymorphic, having only one locus 

 which had A 1 , A, and B alleles (Figure 11, Table 

 11). 



No discernible variation in the frequency or 

 kinds of phenotypes found was observed between 

 rex sole collections from off Astoria (northern), 



TABLE 8. — Results of electrophoretic tests of muscle tissue samples from 145 rex 

 sole collected off Oregon, April 1973. 



1 AAT (aspartate aminotransferase); LDH (lactate dehydrogenase); PGM (phospho- 

 glucomutase); TO (tetrazolium oxidase). 



2 Analysis of muscle proteins was nonspecific, with 6 anodal ( + ) bands and 1 cathodal ( -) band 

 found. 



58 



