mock fish before in irradiation studies in which we 

 either embedded the inoculum evenly throughout 

 the gelatin disc or smeared the same size inoculum 

 on one surface of the gelatin disc (Green and 

 Kaylor 1977). The method might be extended to 

 other applications where some detail or specific 

 effects are to be elucidated. 



From Figure 1 it is obvious that 1% calcium ions 

 negate the effect of 0.1% EDTA and reduce the 

 effect of 1% EDTA. An improved effect is noticed 

 when 10 ppm iodophor is coupled with 0.1% 

 EDTA, and this was somewhat expected. 



The implied conclusion is that 1% EDTA em- 

 bedded in ice, free of divalent ions, will reduce the 

 outgrowth ofPseudomonas spoilage organisms on 

 iced fish and that the inhibitory effect of 0.1% 

 EDTA combined with 10 ppm iodophor is even 

 greater. The expected results obtained with the 

 mock fish supports their reliability for the in- 

 tended use, but it is not suggested for use as a 

 substitute for efficacy tests. Therefore, conculsions 

 regarding the effectiveness of inhibitory additives 

 for any specific substrate must ultimately be de- 

 rived from conventional efficacy tests. 



Literature Cited 

 Green, J. H., and J. D. kaylor. 



1977. Variations in the microbial log reduction curves of 

 irradiated cod fillets, shrimp and their respective homo- 

 genates. Appl. Environ. Microbiol. 33:323-327. 

 LEVIN, R. E. 



1967. The effectiveness of EDTA as a fish preserva- 

 tive. J. Milk Food Technol. 30:277-283. 

 WINARINO, F. G., C. R. STUMBO, AND K. M. HAYES. 



1971. Effect of EDTA on the germination of and outgrowth 

 from spores of Clostridium botulinum 62-A. J. Food Sci. 

 36:781-785. 



John H. Green 



Northeast Fisheries Center Gloucester Laboratory 

 Present address: Department of Food Science 

 Cornell University 

 Ithaca, NY 14853 



LOUIS J. RONSIVALLI 



Northeast Fisheries Center Gloucester Laboratory 

 National Marine Fisheries Service, NOAA 

 P.O. Box 61 

 Gloucester, MA 01930 



REPRODUCTIVE CYCLE OF 



THE PINK SURFPERCH, 



ZALEMBIUS ROSACEUS (EMBIOTOCIDAE) 



Embiotocids received early attention from 

 biologists (e.g., Eigenmann 1892) partly because 

 of the viviparous mode of reproduction displayed 

 by fishes of this family. The pink surfperch, 

 Zalembius rosaceus (Jordan and Gilbert), is one of 

 the' lesser known members of this group. What is 

 most distinctive about Z. rosaceus as compared 

 with other embiotocids is the timing of the various 

 events of its annual reproductive cycle. The pur- 

 pose of this report is to describe this cycle. 



Materials and Methods 



Specimens were collected off the coast of south- 

 ern California at depths ranging from 27 to 33 m. 

 Samples were taken from Redondo Beach, Los 

 Angeles County, to San Clemente, Orange 

 County, Calif. Monthly collections were obtained 

 from May 1972 to September 1973 and January 

 and March 1977. Collections were made using 

 otter trawls from the Occidental College RV Van- 

 tuna and from the RV Fury II, operated by the 

 Orange County Board of Education. Specimens 

 from July, August, and September 1973 were pro- 

 vided by the Southern California Coastal Water 

 Research Project. Specimens were also examined 

 in the ichthyology collection of the Los Angeles 

 County Museum of Natural History. 



The fish were preserved in 10% Formalin. 1 

 Gonads were embedded in paraffin. Histological 

 sections were cut at 8 /jltti and stained with iron 

 hematoxylin followed by eosin counterstain. 

 Gonads were sectioned from the following num- 

 bers of females: January (7), February (4), March 

 ( 11), April (6), May (5), June (10), July (1), August 

 (15), September (18); October (3); December (6); 

 and from 85 males, as shown in Table 1. Sectioned 

 material was collected in 1973 except that for 

 May, June, October, and December 1972. 



Results and Discussion 



The gonadal morphology and histology of Z. 

 rosaceus closely resembles that of the embiotocids 

 Cymatogaster aggregata as described by Eigen- 



1 Reference to trade names does not imply endorsement by the 

 National Marine Fisheries Service, NOAA. 



882 



