Graves et al Biochemical genetics of Paralabrax 



61 



agarose gels (0.8-1.2%) and vertical polyacrylamide 

 gels (3.5-5.0%) at 3 volts/cm. 



The Southern transfer and hybridization protocols of 

 Maniatis et al. (1982) were followed. Hybridization 

 probe mtDNA (purified Pamlahrnx mtDNA) was nick 

 translated with biotinylated dATP using the proce- 

 dures of the BRL Nick Translation System. Visualiza- 

 tion of the fragments followed the procedures included 

 with the BRL BluGene Gene Detection Kit. 



The mean mtDNA nucleotide sequence divergence 

 between the three Paralahrnx species was calculated 



Reference to trade names does not imply endorsement by the Na- 

 tional Marine Fisheries Service, NOAA. 



from the numl^er of shared fragments using the algo- 

 rithm of Nei and Tajima (1983). 



Results 



In the electrophoretic analysis, 43 presumptive gene 

 loci could be resolved in all three species (Table 1). The 

 genetic interpretation of observed banding patterns 

 was guided by expectations based on known patterns 

 of tissue specificity of expression and subunit composi- 

 tion of enzymes in fishes and other vertebrates. Discus- 

 sion of banding patterns for each enzyme system can 

 be found in Waples (1986). Twenty-two loci were mono- 

 morphic (fixed for the same allele) in all individuals 



