NOTES Wilson and Tringali: Isolation and visualization methods for mtDNA studies of clupeids 



615 



earliest life-history stages of these and other important 

 pelagic species. 



Acknowledgments 



This research was supported primarily under NOAA 

 Grant #NA89WC-H-MF028 administered under the 

 Marine Fisheries Initiative (MARFIN) Program for the 

 Gulf of Mexico and faculty start-up funds from the 

 University of South Florida. We thank Dr. John Paul 

 and Lisa Cazares, Department of Marine Science, 

 University of South Florida (USF), for introducing us 

 to the Hoechst 33258 fiuorochrome, and Scott Pichard, 

 also of USF, for assisting with fluorometric quantifi- 

 cations. 



Le Pecq, J.B., and C. Paoletti 



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1982 Molecular cloning. A laboratory manual. Cold Spring 

 Harbor Lab., Cold Spring Harbor, NY, .545 p. 

 Paul. J.H., and B. Myers 



1982 Fluorometric determination of DNA in aquatic 

 microorganisms by use of Hoechst 33258. Appl. Environ. 

 Microbiol. 43:1393-1399. 



Citations 



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