Seeb et a\ : Genetic variation in Panulirus marginatus from the Hawaiian Islands 



715 



each allelic class. Shaklee and Samollow (1984) found 

 no heterogeneity between year classes for any loci ex- 

 cept EstD. and thus they report pooled frequencies. 

 They provide annual frequency estimates for EstD for 

 1979 and 1980. 



We also compared the 1987 data sets from Maro 

 Reef and Necker Island using chi-square contingency 

 analysis. 



Results 



Allozyme analysis 



All loci previously observed to be polymorphic were 

 also found to be variable in the new samples, with the 

 exception oi EstD (Table 1). Allele classes observed by 

 Shaklee and Samollow (1984) and not observed in this 

 study were Est3(f), EstDtJ), and EstD(s). We observed 

 Gpi(H5) in both populations: this allele was not previ- 



ously reported. Additionally, when analyzing Tpfpl 

 allozymes, we detected a Tpep3(9It) allele in a single in- 

 dividual from Maro Reef which was not observed 

 previously. Isozyme patterns agreed with descriptions 

 provided in the earlier study. There were no indications 

 of non-genetic variation at the esterase or any of the 

 other loci. 



Genotypic frequencies at all loci fit the expectation 

 of Hardy-Weinberg equilibrium with the exception of 

 EstS in Maro Reef (P = 0.041) and Mpi in both popu- 

 lations. Mpi has an unusual distribution of alleles 

 between the two sexes (Shaklee 1983). Males always 

 carry at least one slow allele, while females rarely have 

 this variant. The common and slow variant alleles 

 were pooled for the statistical analyses, and only the 

 frequency of the fast allele is reported. With pool- 

 ing, a test for fit to Hardy-Weinberg is possible, and 

 no significant deviation from Hardy-Weinberg was 

 observed. 



