NOTE Renshaw et al,: Microsatellite multiplex panels for genetic studies of Lut/onus gnseus and Lut/onus synogris 



439 



of deviation from Hardy-Weinberg equilibrium expecta- 

 tions was significant (P= 0.009) before Bonferroni cor- 

 rection. One locus, Lea 91, exhibited a one base-pair 

 shift (rather than the two base-pair shifts expected 

 from dinucleotide repeats) in samples of lane snapper; 

 this difference in shift could generate scoring problems 

 in future genotyping efforts. 



The costs of large genotyping projects can be substan- 

 tially reduced through the combination of multiple loci 

 in single PCR amplifications (Renshaw et al., 2006). 

 The reduction in costs could enable researchers to in- 

 crease sample sizes, possibly allowing for the inclusion 

 of additional year classes for temporal studies or could 

 extend a proposed sampling range to include more of 

 the geographic distribution of a species. The multiplex 

 panels and simplex reactions developed in the present 

 study will provide critical tools for future population- 

 level genetic studies designed to identify fishery man- 

 agement units and to monitor changes in genetic effec- 

 tive population size for both gray and lane snappers. 



Acknowledgments 



We thank the crew of the RV Tommy Munro (Florida 

 Fish and Wildlife Research Institute) and M. Murphy, B. 

 Mahmoudi, and R. Lehnert for assistance in sampling, 

 and C. Bradfield and D. Ebelt for assistance in the labo- 

 ratory. Work was supported by the Marfin Program of 

 the U.S. Department of Commerce (Grant NA04-NMF- 

 4330074), and by the Texas Agricultural Experiment 

 Station (Project H-6703). This article is number 54 in 

 the series "Genetic Studies in Marine Fishes" and is 

 contribution number 147 of the Center for Biosystematics 

 and Biodiversity at Texas A&M University. 



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