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Fishery Bulletin 105(3) 



Results and discussion 



A total of 14 microsatellites (11 in three multiplex panels 

 and 3 in simplex reactions) were optimized for gray snap- 

 pers, and 15 microsatellites (in three multiplex panels) 

 were optimized for lane snappers. The multiplex and 

 simplex formats are presented in Table 1 and include for 

 each microsatellite the fluorescent label (Applied Biosys- 

 tems, Foster City, CA), primer quantity, PCR protocol, 

 and optimized annealing temperatures. Genotype sum- 

 mary data from 28 individuals (in both species) are given 

 in Table 2; data for each microsatellite include number 

 of alleles, size range of alleles detected, expected and 

 observed heterozygosity, and probability of conformity 

 to Hardy-Weinberg equilibrium expectations. 



For gray snappers, the number of alleles per microsat- 

 ellite ranged from two (Lea 43 and Lea 91) to 15 (Ra 6), 

 and expected and observed heterozygosity per microsat- 



ellite ranged from 0.036 {Lea 91) to 0.926 (Ra 6) and 

 from 0.036 (Lea 91) to 1.000 iPrs 260), respectively. 

 Only one microsatellite (Ra 6) deviated significantly 

 (P<0.05) from Hardy-Weinberg equilibrium expecta- 

 tions after sequential Bonferroni correction (Rice, 

 1989). Analysis with Micro-checker (Van Oosterhout 

 et al., 2004) indicated the possible occurrence of null 

 alleles at Ra 6. 



For lane snappers, the number of alleles per micro- 

 satellite ranged from two (Prs 275) to 17 (Prs 248), and 

 expected and observed heterozygosity per microsatellite 

 ranged from 0.036 (Prs 275) to 0.938 (Prs 248) and from 

 0.036 (Prs 275) to 0.893 (Prs 248 and Ra 1), respective- 

 ly. None of the 15 microsatellites deviated significantly 

 from Hardy-Weinberg equilibrium expectations after 

 sequential Bonferroni correction (Rice, 1989). Analysis 

 with Micro-checker indicated the possibility of null al- 

 leles at one microsatellite (Lea 20) where the probability 



