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Maria Vernet 



John R. Hunter 

 Southwest Fisheries Center La Jolla Laboratory 

 National Marine Fisheries Service, NOAA 

 P.O. Box 271 

 La Jolla, CA 92037 



Russell D. Vetter 



Marine Biology Research Division 

 Scripps Institution of Oceanography 

 University of California San Diego 

 La Jolla, CA 92093-0202 



Institute of Marine Resources 

 Scripps Institution of Oceanography 

 University of California San Diego 

 La Jolla, CA 92093-0202 



EXTRACTABLE LIPOFUSCIN IN LARVAL 

 MARINE FISH 



The "age pigment", lipofuscin, is thought to be a 

 biochemically heterogeneous byproduct of the 

 peroxidation of polyunsaturated lipids which ac- 

 cumulates in dense, intracellular inclusions 

 called ceroid bodies. The material has been stud- 

 ied both microscopically and biochemically in tis- 

 sues of several species (Miquel et al. 1977; Shi- 

 masaki et al. 1980; Dowson 1982), and some 

 portion of it is quantitatively extractable with 

 organic solvents (Fletcher et al. 1973). 



Flies prevented from flying by putting baffles 

 in the bottles in which they were raised, accumu- 

 lated lipofuscin (as assessed by solvent extrac- 

 tion) more slowly than did free-flying flies but 

 had a longer lifespan, so that at the ends of the 

 respective lifespans the body contents of lipofus- 

 cin were similar in the two groups (Sohal and 

 Donato 1978). Extractable lipofuscin thus ap- 

 pears to accumulate as a function of cumulative 

 oxidative metabolism; it could be an indicator of 

 physiological (rather than strictly chronological) 

 age. 



Additionally, if lipofuscin represents an inte- 

 gral of oxidative metabolism since birth and 

 weight represents an integral of growth over the 

 same period, the ratio of lipofuscin to organic 

 weight should be proportional to the reciprocal of 

 cumulative net growth efficiency [K2 = growth/ 

 assimilation = growth/( growth + respiration), 

 hence I/K2 = 1 + respiration/growth]. 



Ettershank (1984a) introduced the fluoromet- 

 ric measurement of extractable lipofuscin as a 

 measure of physiological age in growing marine 

 crustaceans, based on the work with insects, and 

 (1984b) recommended a simple method for rou- 

 tine use in marine work. He also argued (without 

 presenting extensive evidence) that preservation 



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