56 



Fishery Bulletin 97(1), 1999 



Table 2 



DNA Sequences used to predict restriction digests. Boxes surround primer sequences used in PCR amplification (A=adenine, 

 C=cytosine, G=guanine, T=thymidine, Y=C or T). A vertical line is drawn between the end of the cj^ochrome 6 sequence and the 

 beginning of the threonine tRNA sequence. See Table 1 for description of samples. 



5' Cb3RL -> 



Cal-A 

 Cbr-A 

 Cbr-B 

 Cfa-A 

 Cle-A 

 Cli-A 

 Cob-A 

 Cpl-A 

 Cpl-B 

 Cpl-C 

 Gcu-A 

 Gcu-B 

 Nbr-A 

 Sle-A 

 Smo-A 



Cal-A 

 Cbr-A 

 Cbr-B 

 Cfa-A 

 Cle-A 

 Cli-A 

 Cob-A 

 Cpl-A 

 Cpl-B 

 Cpl-C 

 Gcu-A 

 Gcu-B 

 Nbr-A 

 Sle-A 

 Smo-A 



TCTCCTATTCTCTATCTTCATCCTTATATTGGTGCCCCTCCTCCACACCTCCAAACAACGAAGTACCATCTTCCGACCCA 

 C T..T C A..C..T T T 



. . .T. .T 



'. .C . 

 . .C. 

 . .G 



. .C. 



C T TC . T 



C . . AG T C . 



C C..T C. 



.A. .C. 

 . . . .T. 

 .A. . T. 

 . AA . T . 

 .A. .T. 



. . .A. .T. 

 . C. AA.T. 

 •C.A. .C . 



. C .G. .C . 



C . . TT . 



• C.A. 



• C.A. 



.T..A..A..T A. 



. C . TTTA. ..T T A. 



. .A. 

 . AAC 



.AC 

 .AC 



continued 



men from the Gulf of Mexico (Cpl-B) differed from 

 that of the specimen from Hawaii (Cpl-C) by three 

 base-pair substitutions. The sequence from the speci- 

 men from Hawaii (Cpl-C) differed from that of Martin 

 and Palumbi (1993) by one base-pair (and which af- 

 fected thefo^I restriction site). Whether the single base 

 pair difference between our specimen from Hawaii and 

 the one reported in Martin and Palumbi ( 1993) repre- 

 sents an uncommon polymorphism in sandbar sharks 

 from the Pacific or an error in reading the original se- 

 quence cannot be determined. We examined restriction 

 digestions from three sandbar sharks collected near 

 Hawaii and all possessed the Fokl restriction site. 



We also found a populational difference between 

 spinner sharks (C. brevipinna) collected from the 

 North Atlantic (including the Gulf of Mexico) and 

 from the Pacific coast of Australia. Initially, we se- 



quenced a spinner shark from Australia and pre- 

 dicted that Rsal would not cut the diagnostic frag- 

 ment. We restricted fragments from seven spinner 

 sharks, two from Australia, and five from the U.S. 

 Atlantic and Gulf of Mexico coasts. Although frag- 

 ments from the two spinner sharks from Australia 

 were not cut with Rsa I, each of the five specimens 

 from the Atlantic produced fragments of 251 and 144 

 bp. We sequenced one specimen from the Atlantic (spin- 

 ner B) and found two nucleotide substitutions, includ- 

 ing one that resulted in the restriction site difference 

 between spinner sharks from the Atlantic and Pacific. 

 We detected numerous differences between restric- 

 tion patterns predicted from the sequence of Martin 

 and Palumbi ( 1993 ) for tiger shark collected near Ha- 

 waii and our tiger sharks collected from the Gulf of 

 Mexico (Atlantic) and Hawaii and Australia (Pacific). 



