NOTE Mollet et al : Re identification of a lamnid shark embryo 



867 



between the first dorsal fin origin and the pectoral fin free 

 rear tip (PD1-PRT = PD1-(PP1 + PIB + PID). IfPlBor 

 PlI were not available for shortfin makos, we estimated 

 (PlB + PlDas ll%ofTL(orTOT). 



Tissue samples for DNA sequencing 



Tissue samples were taken from the gill slits, the oral 

 cavity, and the caudal peduncle. The samples were stored 

 at room temperature in Wheaton polypropylene vials 

 in QS^f ethanol. DNA was extracted from the samples 

 and polymerase chain reaction (PCR) amplification and 

 sequencing were attempted but did not yield useful 



results (Bernardi''). This was likely due to initial fixing 

 of the specimen in formalin (which destroys DNA) before 

 transferal to ethanol. 



X-ray analysis 



For vertebral counts, we used radiographs taken initially 

 with a Siemens Triselenix 750 (in Milan) and later with 

 a Shimadzu R20 computerized x-ray machine for high- 



^ Bernard!, G. 1996. Personal commun. Dept. of Biology. Uni- 

 versity of California, Santa Cruz, CA 95064. 



