Bostrom et a\ Hybridization between two serranids, Ccphalopholis futva and Paranthias furafer 657 



for which it displayed two alleles diagnostic of C. fulva. 

 Cephalopholis cruentata was distinguished from C. fulva 

 and P. furcifer at the ICDH-S* locus and was eliminated as 

 a potential parent species. 



Nuclear intron regions 



An actin intron appro.ximately 450 base pairs in length 

 was amplified. The region was surveyed with 14 restric- 

 tion enzymes, of which Hint I showed a genetic difference 

 between species. After digestion with Hint I, all C. fulva 

 demonstrated allele A, and all P. furcifer diplayed allele B 

 (Tables 6 and 7). All fifteen putative hybrids were hetero- 

 zygous for both alleles. 



The second intron region of the S7 ribosomal protein, 

 which was approximately 1200 base pairs in length, was 

 screened with thirty-five enzymes. Two enzymes, Dra I 

 and Alu I, demonstrated differences between P. furcifer 

 and C. fulva. Paranthias furcifer and C. cruentata both 

 exhibited allele D after digestion with Dra I (Tables 6 and 

 7 ). Cephalopholis fulva was variable at this locus — fifty in- 

 dividuals were homozygous for allele A, three homozygous 

 for allele E, and four heterozygous for alleles A and E. All 

 fifteen putative hybrids were heterozygous at this locus 

 with one of the C. fulva alleles (A or E), and the P. furcifer 

 allele (D). Digestion of the second intron in the S7 region 

 hy Alu I produced a large number of small fragments that 

 were not easily interpreted and the data were not used 



