Olsen et a\ An examination of spatial and temporal genetic variation in Jlieiagia chalcogiamma 



755 



Table 2 



BiifTors and tissues used to resolve enzyme-coding loci in walleye pollock. Enzyme nomenclature and Enzyme Commission (EC I 

 number follow lUBNC ( 1984); locus nomenclature follows Shaklee et al., ( 1990). 



Enzyme or protein 



EC number 



Locus 



Tissue' (buffer^) 



Aspartate aminotransferase 



Adenosine deaminase 



Aconitatc hydratase 



Alanine aminotransferase 

 Creatine kinase 

 Fumarate hydratase 



2.6.1.1 



3.5.4.4 



4.2.1.3 



2.6.1.2 

 2.7.3.2 

 4.2.1.2 



sAATl* L(AC6.5) 



sAAT-2* H(AC6.1), M{AC6.1) 



niAAT-l* M(AC6.1) 



ADAl* L(AC6.5) 



ADA-2* L(AC6.5) 



AH-1* L(AC6.5) 



AH-2* H(AC6.5), M(AC6.1, AC6.5I, L(AC6.5) 



ALAT* M(TBE) 



CK-A* M(TBE) 



FH* M(ACEN7.0) 



were size fractionated on 3% agarose gels, stained with 

 ethidium bromide, and visualized and photographed un- 

 der ultraviolet light (312 nm). Fragment sizes as small 

 as 50 bp were estimated by comparison with 100 bp and 



250 bp ladders on each gel, A composite haplotype was 

 generated for each individual by recording the presence or 

 absence of restriction sites for all restriction enzymes and 

 mtDNA segments (Lansman et al,, 1981), The length of 



