FISHERY BULLETIN: VOL. 87, NO. 3, 1989 



Table 1. — Starch-gel electrophoretic protocols used to reveal allozyme variation. 



'Buffers A, B, C, and D as described by Tracey et al. (1975); buffer E is the lithium borate discontinuous buffer system 2 of Selander et al. 

 (1971). 

 ^a-naphthyl butyrate and p-naphthyl acetate as substrates. 

 'L-glycyl-leucine as substrate. 

 "L-leucyl-glycyl-glycine as substrate. 



^A 1 :1 mixture of L-leucyl-valine and L-leucyl-tyrosine as substrates. 

 ^Known as malic enzyme 

 ''L-phenylalanyl-proline as substrate. 

 'Stained with Coomassie blue. 

 ^Usually scored on gels stained for HBDH. 

 '°Di- and tripeptidases counted as one enzyme in totals. 



enter (4.000), F-to-remove (3.996), and prior 

 probabilities (equal). 



Engraulis Mordax 



Collections 



Samples of 48 anchovies were obtained from 

 each of nine mid water trawl stations (Fig. 1) 

 occupied between the nights of 24 January and 1 

 February 1982, by CalCOFI cruise 8202 aboard 

 the RV David Sta7T Jordan of NOAA Southwest 

 Fisheries Center, La JoUa. CalCOFI grid coor- 



dinates (CalCOFI Atlas No. 1, 1963) for these 

 collections and place names assigned to them for 

 convenience were as follows: 1) Half Moon 

 Bay, 63.3:51.0; 2) Half Moon Bay, 63.3:52.0; 3) 

 Santa Cruz, 66.7:49.0; 4) Santa Cruz, 66.7:50.0; 

 5) Santa Barbara, 81.3:42.4; 6) Point Dume, 

 85.0:44.0; 7) Santa Monica Bay, 85.8:34.0; 8) 

 Santa Monica Bay, 85.8:36.0; 9) Santa Monica 

 Bay, 85.8:41.0. Whole fish were frozen individ- 

 ually aboard ship at - 70°C and then packaged in 

 plastic bags labeled by locality. Frozen packages 

 were shipped by air to the Bodega Marine 



656 



