SHAKLEE and SAMOLLOW: GENETIC VARIATION IN SPINY LOBSTER 



MPI 







EST 



:• 



-103 

 -100 

 -95 



• * 



MM, 



m_f_f "2 £!2 HI £!} m H5 H} 

 ssmssmsmss 



rn rn _t_ ^i rn nn rn £n jti nnrn 

 mmsrns mmrnmmm 



PEP 



mmm s s rnmrnmm 



PGM 



UMB 



mmm_fmmm_f_fmm 

 mmmmmmmmmmm 



GPI 



± ± 



s f 



±±±±111111 11111 



f f f f f f f f f f f f m f f 



FIGURE 2. — Isozyme patterns of spiny lobster, Panulirus marginatus. MPI = 

 mannosephosphate isomerase, EST = esterase-3, PEP = peptidase-2, PGM = 

 phosphoglucomutase, UMB = umbelliferyl esterase ( = EST-D), GPI = glucose- 

 phosphate isomerase. Alleles are indicated at the right of each gel. Scoring of 

 individual genotypes (by allelic class) is indicated at the bottom of each gel. Note 

 that the sample origin is at the bottom and the anode is toward the top. 



of the rarity of variant alleles, and therefore, of all 

 but the common homozygote at all loci except 

 Pep-1 and Mpi, tests of Hardy- Weinberg equilib- 

 rium were not very robust; most genotypic classes 

 had to be pooled to obtain adequate numbers of 



expected genotypes. In all cases when there were 

 three or more alleles, these were pooled into two 

 groups; a group consisting of only the most com- 

 mon allele and a group including all other alleles. 

 Furthermore, in the case of Mpi the unusual na- 



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