FISHERY BULLETIN: VOL. 82, NO. 4 



TABLE 4. — Frequencies of the more abundant allele at each 

 polymorphic locus in pink snapper of different sizes (ages). 



' Abbreviations of loci and alleles as in Table 3; W = number of fish success- 

 fully scored. 



Consisting of fish from Maro Reef and French Frigate Shoals (FFS) and fish 

 from Necker with known fork length. 



TABLE 5. — Frequencies of the more abundant allele at each 

 polymorphic locus in pink snapper collected at different times. 

 — = no data because tissues had been stored in brine (see Mate- 

 rials and Methods). 



'Abbreviations of loci and alleles as in Table 3; N = number of fish success- 

 fully scored. 



sample. The other three loci exhibited no such 

 clear trends.in changing allele frequency with size 

 (age). These analyses indicate that allele frequen- 

 cies at some loci (Adh and Ldh-C) in the 

 opakapaka may exhibit important changes re- 

 lated to size (age) and caution against an uncriti- 

 cal analysis of pooled data. 



It was possible to analyze temporally subdivided 

 samples from Maro Reef and FFS to see whether or 

 not significant changes in allele frequency at a 

 location were occurring through time. The allele 

 frequencies characteristic of these temporal sam- 

 ples are shown in Table 5. Unfortunately, in order 

 to obtain adequate sample sizes it was necessary 

 to include specimens of all sizes (ages) in these 

 samples. Of the 19 sets, only Umb in the May 1980 

 sample from FFS was significantly out of Hardy- 

 Weinberg equilibrium (* 2 i = 6.14; P < 0.025), 

 exhibiting an excess of heterozygotes. Of the 10 

 contingency x 2 tests between successive samples, 

 only that for Ad at Maro Reef was statistically 

 significant (x 2 t = 5.80; P < 0.025) and that in- 



volved fish of significantly different sizes in the 

 two samples (Fig. 2). Although this temporal 

 analysis is not as complete as one might wish, it 

 does suggest that allele frequencies were reason- 

 ably constant over the 2Vfe yr period of the present 

 study 



Given that allele frequencies remained rela- 

 tively constant through time but exhibited sig- 

 nificant changes (at two loci) associated with fish 

 size (age), a final analysis of the data was con- 

 ducted. This involved fish from all five locations 

 but only fish of 300-550 mm FL (about 2-6 yr old; 

 see Ralston and Miyamoto 1983). The allele fre- 

 quencies characteristic of these samples are pre- 

 sented in Table 6. In all cases (5 loci x 5 locations 

 = 25 tests) the data were in agreement with 

 Hardy- Weinberg equilibrium expectations. Even 

 more importantly, the outcomes of all five x 2 con- 

 tingency tests (involving all locations) were non- 

 significant. The results reinforce the earlier con- 

 clusions that no among-locality genetic differen- 

 tiation exists in pink snapper throughout the 



TABLE 6. — Frequencies of the more abundant allele at each poly- 

 morphic locus in pink snapper between 300 and 550 mm FL. 



'Abbreviations of loci and alleles as in Table 3; N = number of fish successfully 

 scored. 

 2 Including 25 fish from Kauai. 



3 Consisting of fish from Maro Reef, French Frigate Shoals, and Necker. 

 "Consisting of fish from Kauai, Molokai. and Hawaii. 



708 



