SHAKLEE and SAMOLLOW: GENETIC VARIATION IN A DEEPWATER SNAPPER 



RESULTS 



Forty-four presumed gene loci encoding 29 dif- 

 ferent enzymes were surveyed in a total of 716 pink 

 snapper collected at six localities over a 2V2-yr 

 period. Thirty-nine of these loci were monomor- 

 phic in the first 100 animals screened (21 from 

 Maro Reef, 44 from FFS, and 35 from Necker). 

 These enzymes, which were not studied further, 

 were aspartate aminotransferase (2 loci), acid 

 phosphatase, adenosine deaminase, adenylate 

 kinase, alkaline phosphatase, alanine amino- 

 transferase (2 loci), catalase, creatine kinase (3 

 loci), esterase (2 loci), glyceraldehyde-3-phosphate 

 dehydrogenase (2 loci), glutamate dehydrogenase, 

 glucosephosphate isomerase-A, glycerol-3- 

 phosphate dehydrogenase (2 loci), hexosediphos- 

 phatase (2 loci), isocitrate dehydrogenase, 

 lipoamide dehydrogenase (= diaphorase), lactate 

 dehydrogenase (A and B), malate dehydrogenase 

 (2 loci), malate dehydrogenase-NADP + (= malic 

 enzyme), mannosephosphate isomerase, peptidase 

 (4 loci), peroxidase, phosphogluconate dehy- 

 drogenase, phosphoglucomutase, pyruvate 

 kinase, superoxide dismutase, and xanthine 



dehydrogenase. The remaining five loci were 

 polymorphic and the conditions for their analysis 

 are summarized in Table 2. Since only 5 out of 44 

 loci in pink snapper were found to be polymorphic, 

 P.95 is estimated to be 0.114 in this species. 



Zymogram patterns showing the commonly ob- 

 served phenotypes for 4 of the 5 polymorphic en- 

 zymes in pink snapper are shown in Figure 1. The 



TABLE 2. — Polymorphic enzymes in pink snapper, Pris- 

 tipomoides filamentosus: Characteristics and conditions for 

 analysis. 



'Presumed structure based on isozyme banding pattern in heterozygotes 

 (see text). 



2 EDTA-boric acid-Tris pH 8.6 buffer of Boyer et al. (1963). 



3 Citricacid-aminopropyldiethanolamme (gel = pH 7.5. electrodes = pH 7.2) 

 buffer of Clayton and Tretiak (1972). 



"Tris-citric acid pH 7.0 buffer (buffer 1 of Shaw and Prasad 1970). 



5 Tris-citric acid pH 7.0 buffer (same as TC-1 but gel buffer is a 1 :6.5 dilution of 

 the electrode buffer). 



LDH 



IDDH 



•I06 

 •IOO 



203 



i 



iiiiiiiiii 



ssssss sss f 



UMB 



i 1 i i i 



s s s f s 



1111 

 s s s s 



ADH 



iiilLlliik 



s f f f f S S f f s 



FIGURE 1. — Isozyme patterns of pink snap- 

 per, Pristipomoides filamentosus. LDH = 

 lactate dehydrogenase, IDDH = L-iditol de- 

 hydrogenase (= sorbitol dehydrogenase), 

 ADH = alcohol dehydrogenase, UMB = um- 

 belliferyl esterase. Alleles are indicated at 

 the right of each gel. Scoring of individual 

 genotypes (by allelic class) is indicated at the 

 bottom of each gel. Note that the sample ori- 

 gin is at the bottom and the anode is toward 

 the top except for ADH where the origin is at 

 the top (and the cathode is toward the bot- 

 tom). 



705 



