■'^•.C.' 

 ••*.• ^ 







**^^f. 



■•^- 



^^J»*. 



Figure l. — Sagittal sections of livers of northern anchovy larvae from the laboratory, showing the three grades based on intensity of 

 the PAS staining reaction. Except for the nuclei, and occasional portions of other organs, black and the darker shades of grey represent 

 PAS-positive red material presumed to be glycogen. All photomicrographs were taken and processed in the same way All were taken at 

 787 X. A. 5.5 mm larva, fed, graded High. B. 5.0 mm larva, starved, graded Medium. C. 6.1 mm larva, starved, graded Lxiw. D. 5.2 mm 

 larva, fed, graded High. The first three were fixed in Bouins fluid, but the fourth was fixed in l(Ff alcoholic Formalin and showed a very 

 intense staining reaction. 



Table 3. — X^ and mean contingency square iX'^ In) values for 

 the association between PAS glycogen rating and food treatment 

 for the arrays of larvae shown in Table 1 ( laboratory i and Table 2 

 (ocean). Each set of values is for a 2 x 3 contingency table with 

 I n ) larvae and 2 df. In each group, small lai-vae are those below, 

 and large larvae those above, the median standard length. 



that only for the lower part of the length range is 

 there a meaningful association between treat- 

 ment (histological characterization of the samples 

 from which larvae were drawn) and liver glycogen 

 rating. As already mentioned, the relevant asso- 



ciation is High ratings with samples of generally 

 robust histological condition, and Low ratings 

 with samples of generally emaciated condition. 

 The mean square contingency values (X^/n) indi- 

 cate the extent to which the association for small 

 larvae from the ocean is weaker than those from 

 the laboratory. 



The dozen larvae from the offshore samples, not 

 included in the above analysis of inshore larvae, 

 showed relatively high liver glycogen ratings. 

 They were almost equally divided among three 

 tows, each of which was taken between 0300 and 

 0400 h on a different day and contained larvae in a 

 different part of the size range. There were no Low 

 liver glycogen ratings among them. Ratings 

 were largely Medium for the small to medium 

 standard lengths (3.4-7.8 mm) and High for the 

 largest larvae (8.0-9.2 mm). These High ratings, 

 moreover, represent the most intensely PAS 



809 



