463_ 



STATE OF WASHINGTON DEPARTMENT OF FISHERIES - EXHIBIT A 



2 point out that the work of Professor Breese referred to by 



3 Mr. Benson was carried out with "stripped" rather than 



4 spawned oyster eggs. The use of "stripped" eggs for any 



6 sort of molluscan larvae work has been generally discredited 

 g by Dr. Loosanoff and his co-workers as well as most other 



7 researchers studying and growing bivalve larvae. Further, 

 g we feel that the oyster larvae bloassays reported by the 

 9 FWPCA were conducted at a temperature considered to be quite 



IQ ideal by marine biologists working with oyster larvae. 



11 Considering Mr. Haydu's statements on the 



12 oyster larval bioassay tests, we agree that differences 



13 exist between response levels of various forms (Mr. Haydu 

 1^ considers shrimp, fish and oysters) to some toxicants, and 



concur that safe levels for one species may be lethal to 

 another. We also agree in principle with his comments re- 



15 

 16 



ly gardlng the use of "reference animals" to determine relative 



18 

 19 



toxicities. We would extend this particular point to in- 

 clude the fact that if any reference animal is antagonized 



20 in any way by the material being tested, such material not 



21 be discharged to a water course until it is proven nontoxic . 



22 We feel that an "appropriate margin of safety" should be 



23 applied by the FWPCA with respect to oyster larvae. 



24 Also even though species of shellfish native 



25 to the area (Everett) have not been used as bioassay animals. 



