TABLE OF CONTENTS 



Page 



PREFACE 1 



LIST OF TABLES 7 



LIST OF FIGURES 8 



I: INTRODUCTION 21 



II: MATERIALS AND METHODS 33 



Experimental Design 33 



Experimental Animals 33 



Laboratory Holding Procedure 34 



Temperature Acclimation Procedure 35 



Preparation of Salinity Media 35 



Preparation of Deviated Ion Media 37 



Behavior 38 



Behavior in Media of Deviated Ion Ratios 38 



Blood Sampling 40 



Blood Analyses 41 



Osmotic concentration 41 



Blood chloride 41 



Other electrolytes 41 



Determination of Oxygen Consumption 42 



Flow-through respirometry 42 



Oxygen consumption in media with deviated ions . . 46 



Statistics 46 



III: RESULTS 48 



Effect of Salinity and Temperature on Behavior 



and Survival 48 



Effect of salinity change 49 



Effect of temperature background 52 



Effect of salinity and temperature change 53 



Blood Osmoregulation During the Time Course of 



Adaptation 56 



Effect of 25°C acclimation on osmoregulation ... 66 



Effect of 32°C acclimation on osmoregulation ... 67 



Effect of 18°C acclimation on osmoregulation ... 68 



