Canadian Maritimes and indicates that current 

 management practices result in the transfer of 

 PEN and G. hertwigi from one area to another via 

 infected smelt. 



Materials and Methods 



For evidence of PEN, blood samples of 1,412 

 anadromous Osmerus mordax (12.7-27.3 cm total 

 length. TL) were collected at 42 sites from 15 

 coastal smelt streams from Massachusetts to the 

 Canadian Maritimes (Figure 1) between 3 

 November 1976 and 17 November 1977: Kittery, 

 Maine (Spruce Creek); Boothbay, Maine (Hodgdon 

 Cove and Boothbay Harbor); Bath, Maine 

 (Whiskeag Creek); Dresden, Maine (Eastern 

 River); Damariscotta, Maine ( Damariscotta 

 River); Wiscasset, Maine (Montsweag Creek): 

 Warren, Maine (St. George River); Addison, 

 Maine (Harrington River); Winterport, Maine 

 (Penobscot River); Newington, N.H. (Great Bay); 

 Kingston, Mass. (Jones River); Hingham, Mass. 

 (Weir River); Quarryville, New Brunswick, 



Canada (Miramichi River); Portapique, Nova 

 Scotia, Canada (Portapique River); and Oxford, 

 Nova Scotia, Canada (Philip River). 



In addition, blood samples from 256 landlocked 

 O. mordax (7.6-27.0 cm TL) were collected in nine 

 samples from five Maine lakes between 15 Jan- 

 uary and 5 May 1977: Damariscotta (the lake 

 is approximately 10 mi long, begins at Jefferson, 

 Maine, and empties into the Damariscotta River 

 at Damariscotta); Wyman (Bingham, Maine); 

 South Twin and Millinocket ( Millinocket, Maine); 

 and East Musquash (Topsfield, Maine). 



For evidence of G. hertwigi. 1,692 anadromous 

 O. mordax (12.7-27.3 cm TL) were collected in 42 

 samples from 16 localities (the above ISplusCasco 

 Bay, Maine) between 3 November 1976 and 17 

 November 1977. In addition, 254 landlocked O. 

 mordax (7.6-27.0 cm TL) were collected in eight 

 samples from the above five lakes between 15 

 January and 5 May 1977. 



Smelt were obtained from our own catches, from 

 those of fishermen and from Massachusetts Divi- 

 sion of Marine Fisheries personnel. Depending 



W)NTERPORT 

 28% (7/ 25) 



BINGHAM 

 0% (0/25)® 



DRESDEN 

 73% (59/81) 



BATH \ 



65%(135/208)- 



KITTERY, ME 

 37%(I5/41) 



NEWINGTON N, 

 627o(l5/24 



HINGHAM, MAV 

 99%(99/IOO) A 



Ji 



QUARRYVILLE 

 21% (48/ 223 



MILLINOCKET 

 ®®0% (0/77) 



TOPSFIELD 

 0%(0/50)® 



^(^Vaddison ^^ 

 ;^r,'^ioo%(ioo/io(5v' J. 



" ' WARREN \ y 



I07o (40/50) ^ p^ 



DAMARISCOTTA LAk'^ 

 4% (4/104) 

 DAMARISCOTTA RIVER 

 46% (52/114) 



BOOTHBAY 

 50% (56/ I 



-WISCASSET 

 447o (11/25) 



-KINGSTON, MA 

 100 7o 1100/ 100) 



I) 



(•) denotes locations of 

 inland lakes 



Figure l. — incidence and distribution of piscine er>'throcytic necrosis (PEN) in anadromous rainbow smelt from the Canadian 

 Maritimes to Massachusetts and landlocked rainbow smelt from five Maine lakes (two lakes are located at Millinocket). 



504 



