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KEVIN M. HOWE 



David L. STEIN 



Carle. BOND 



Department of Fisheries and Wildlife 

 Oregon State University. Corvallis, OR 97331 



School of Oceanography 



Oregon State University, Corvallis, OR 97331 



Department of Fisheries and Wildlife 

 Oregon State University, Corvallis, OR 97331 



CHANGES IN RIBONUCLEIC ACID, 



DEOXYRIBONUCLEIC ACID, AND PROTEIN 



CONTENT DURING ONTOGENESIS IN 



WINTER FLOUNDER, PStUDUPLllVROSECTES 



AMERICANUS, AND EFFECT OF STARVATION 



Normal development of most embryonic and pro- 

 larval (yolk-sac) teleosts depends on material 

 stored in the yolk for a source of both energy and 

 biosynthetic precursers. After hatching there is a 

 transition period when larvae shift from depen- 

 dence on yolk to an exogenous food supply. The 

 availability of sufficient prey of the proper quality 

 and the ability of larvae to capture and assimilate 

 it are critical to survival during the larval stage. 

 Since differential mortality during the larval 

 stage could be important in determining the 

 year-class size of marine fish, a method for deter- 

 mining the nutritional condition offish larvae in 

 plankton samples could aid in determining larval 

 survival and prediction of subsequent year-class 

 size. In the past, weight-length relationships 

 (Blaxter 1971), morphometric (Ehrlich et al, 

 1976), chemical (Ehrlich 1974a, b), and histolog- 

 ical (O'Connell 1976; Theilacker 1978) methods 

 have been used with varying degrees of success. 

 All four approaches have limitations and diag- 

 nosis of the starving condition in sea-caught lar- 

 vae is difficult, 



Bulow (1970) used RNA-DNA (ribonucleic 

 acid-deoxyribonucleic acid) ratios as indicators 



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