564 



FISHERY BULLETIN OF THE FISH AND WILDLIFE SERVIC5E 



susceptible to furunculosis; the other resistant to 

 this disease — and one strain of brown trout. 

 Since it was found that the disease was caused by 

 a sulfa-resistant type of the pathogen, only the 

 treatment with the antibiotic was effective. 



Testing for di'ug resistance has shown that many 

 strains of A. salmonicida were resistant to sulfona- 

 mides, but sensitive to antibiotics. Therefore, 

 studies were made in order to develop a rapid and 

 reliable field method for the determination of drug 

 sensitivity of A. salmonicida. 



Of all media tested, only the Trypticase Soy 

 agar and the modified Leetown standard furuncu- 

 losis medium can be used for the determination 

 of sensitivity of A. salmonicida to sulfonamides 

 and antibiotics and at the same time for the rapid 

 presumptive test of Griffin. Mueller Hinton agar 

 is excellent for the determination of drug sensi- 

 tivity, but not for the presumptive test of Griffin 

 involving the production of brown pigment. 



A method of the determination of drug sensi- 

 tivity employing media in the form of agar slants 

 and single disks containing the drug is recom- 

 mended for field use. 



A revised and up-to-date method of treatment 

 and control of furunculosis is described. 



LITERATURE CITED 



BuRDETTE, Robin I., LeRoy E. Plank, and William E. 

 Clapper. 



1955. A comparison of four methods of testing sul- 

 fonamide sensitivity and the relation to clinical 

 response in urinary tract infections. Antibiotics 

 and Chemotherapy, vol. 5, pp. 392-397. 

 Deuel, Charles R., David C. Haskell, Donald R. 

 Brockway, and O. R. Kingsbury. 



1952. The New York State fish hatchery feeding 

 chart. Fisheries Research Bulletin No. 3, third 

 edition, 61 pp. New York State Conservation 

 Department, Albany, N. Y. 



Griffin, Philip J. 



1952. A rapid presumptive test for furunculosis in 

 fish. Progressive Fish-Culturist, vol. 14, pp. 74-75. 

 U. S. Fish and Wildlife Service, Washington, D. C. 



Griffin, Philip J., S. F. Snieszko, and S. B. Friddle. 



1953. A more comprehensive description of Bade 

 rium salmonicida. Trans. American Fisheries Soc, 

 vol. 82 (1952), pp. 129-138. 



Outsell, James S. 



1948. The value of certain drugs, especially sulfa 

 drugs, in the treatment of furunculosis in brook 

 trout, Saheliniis fontinalis. Trans, .\merican Fish- 

 eries Soc, vol. 75 (1945), pp. 186-199. 

 Johnson, H. E,, and R. F. Brice. 



1953. Mortality of silver salmon from treatments 

 with sulfamerazine. Progressive Fish-Culturist, 

 vol. 15, pp. 31-32. U. S. Fish and Wildlife Service, 

 Washington, D. C. 

 McCraw, Bruce M. 



1952. Furunculosis of fish. Special Scientific Re- 

 port: Fisheries No. 84, 87 pp. U. S. Fish and Wild- 

 life Service, Washington, D. C. 

 Northey, Elmore H. 



1948. The sulfonamides and allied compounds, 

 xxvii and 660 pp. Reinhold Publishing Co., New 

 York. 

 Snieszko, S. F. 



1954a. Therapy of bacterial fish diseases. Sym- 

 posium, Research on Fish Diseases. Trans. Ameri- 

 can Fisheries Soc, vol. 83 (1953), pp. 313-330. 

 1954b. Advances in the studies of infectious fish 

 diseases. Trans. Meeting Southeastern Fish and 

 Game Commissioners, 3 pp. New Orleans. 

 Snieszko, S. F., and S. B. Friddle. 



1951. Tissue levels of various sulfonamides in trout. 

 Trans. Ameiican Fisheries Soc, vol. 80 (1950), pp. 

 240-250. 



Snieszko, S. F., and P. J. Griffin. 



1955. Kidney disease in brook trout and its treat- 

 ment. Progressive Fish-Culturist, vol. 17, pp. 

 3-13. U. S. Fish and Wildlife Service, Washing- 

 ton, D. C. 



Snieszko, S. F., P. J. Griffin, and S. B. Friddle. 



1952. Antibiotic treatment of ulcer disease and fu- 

 runculosis in trout. Trans. Seventh North .Amer- 

 ican Wildlife Conference, pp. 197-213. 



Snieszko, S. F., and E. M. Wood. 



1955. The effect of some sulfonamides on the growth 

 of brook trout, brown trout and rainbow trout. 

 Trans. American Fisheries Soc, vol. 84 (1954), pp. 

 86-92. 

 Wales, J. H., and William Berrian. 



1937. The relative susceptibility of various strains 

 of trout to furunculosis. California Fish and Game, 

 vol. 23, pp. 147-148. 

 Wolf, Louis E. 



1954. Development of disease-resistant strains of 

 fish. Symposium, Research on Fish Diseases. 

 Trans. American Fisheries Soc, vol. 83 (1953), pp. 

 342-349. 

 Work, Thomas S., and Elizabeth Work. 



1948. The basis of chemotherapy, xvi and 435 pp. 

 Interscience Publishers, New York. 



U. S. GOVERNMENT PRINTJNG OFFICE ; 1957 O — 433457 



