FISHERY BULLETIN: VOL. 84, NO. 4 



diameter. Unknown factors in our laboratory en- 

 vironment resulted in precocious sexual maturation 

 and thus smaller animals. Therefore, we are now 

 evaluating the culture potential of Loligo forbesi, a 

 much larger squid from the eastern Atlantic, since 

 precocious maturation in that species would still 

 result in axons >500 /urn. Preliminary experiments 

 bear out this proposition as we have recently 

 cultured L. forbesi to 140 mm ML and 400 \xm 

 diameter axons. However, L. opalescens would be 

 an excellent model for the giant synapse preparation 

 in which smaller squid are most suitable. Therefore, 

 L. opalescens, with a now substantial amount of 

 culture information, may be a highly suitable species 

 in the United States for providing squid on a con- 

 sistent basis for neuroscience research. Moreover, 

 the recent disappearance of L. opalescens (1983-85) 

 from traditional fishing grounds in California make 

 laboratory culture an attractive alternative for 

 animal supply. 



ACKNOWLEDGMENTS 



We acknowledge funding from DHHS grant 

 RR01024, Division of Research Resources, National 

 Institutes of Health, and from the Marine Medicine 

 General Budget 7-11500-765111 of The Marine Bio- 

 medical Institute, The University of Texas Medical 

 Branch. We especially appreciate the assistance of 

 John W. Forsythe on the rearing experiments and 

 the growth data analyses. We also thank Joseph P. 

 Hendrix Jr. for assistance in rearing and Lea A. 

 Bradford for water analyses and data gathering. 

 Connie Arnold and Joan Holt of the Port Aransas 

 Laboratory, University of Texas, kindly supplied the 

 red drum eggs. We are grateful to Aquabiology 

 (Seibutsu Kenkyusha Publishing Co., Tokyo) for per- 

 mission to reprint several figures. Academic Press 

 Inc. kindly gave us permission to use a modification 

 on Figure 17. Phillip G. Lee kindly read and im- 

 proved the final draft. 



Note: We dedicate this paper to our coauthor and 

 dear friend Dr. Raymond F. Hixon, who passed 

 away on 19 March 1984 as he valiantly fought to 

 recover from chronic myelogenous leukemia. 



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796 



