FISHERY BULLETIN: VOL. 84, NO. 3 



50 100 150 



KILOMETERS 



Project on a'tei IWXjpi 1965 I 



68° 



tf C NMFS Sano, ► 



Figure 1.— Benthic stations where gammaridean amphipods were sampled during the survey. 



ent in a sample, and sometimes more, depending on 

 numbers present, were prepared for histological 

 study. Details of collecting procedures and histo- 

 logical preparation of the amphipods are given by 

 Johnson (1985). Sections were cut at 6 ^m. Stain- 

 ing methods included Harris' hematoxylin and eosin 

 (H&E), the Feulgen reaction, and Alfert and Gesch- 

 wind's (1953) fast-green method for demonstration 

 of basic nuclear proteins. Harris' hematoxylin and 

 eosin is specified because this combination stains 



nuclei of the parasites purple during certain stages. 

 Other hematoxylin solutions, used with eosin, will 

 not necessarily impart the same distinct purple 

 color. Unless otherwise indicated, references to 

 staining properties of the organisms are to H&E- 

 stained specimens. 



OBSERVATIONS 



Thirteen amphipod species were infected with syn- 



606 



