314 BULLETIN OF THE UNITED STATES FISH COMMISSION, 



water) at 9.40 a. m., May 21; 11 a. in., showed signs of weakness, part 

 going to the bottom ; 12.30 p. m., few swimming, but majority were at 

 the bottom and quiet; 1.30 p. m., all were at bottom, many dead; 2.15 

 p. m., all dead. 



4. — One huudred newly hatched sbad were put in 20 ounces of mixt- 

 ure, 25 per cent strength (5 ounces of refuse and 15 ounces of Potomac 

 water) at 9.40 a. in., May 21, 11 a. m., all up and swimming, but a little 

 weak ; 12.30 p. m., about one half on bottom, others weak and spas- 

 modic in their movements; 1.30 p. in., half dead, others dying; 4 p. 

 in., all dead. 



5. — One hundred newly-hatched shad were put in 20 ounces of mixt- 

 ure, 10 per cent strength (2 ounces of refuse and 18 ounces of Potomac 

 water) at 9.40 a. in., May 21 ; 12.30 p. in., but few were affected, major- 

 ity up and swimming but showing slight signs of weakness; 6.30 p. m., 

 50 per cent dead ; 8 p. m., 75 per cent dead ; 7 a. m., 22d, 90 per cent 

 dead ; 4 a. m., 23d, all dead. 



6. — One hundred newly-hatched shad were put in 20 ounces of Poto- 

 mac water at 9.40 a. m., May 21. All were still alive when all were 

 dead in the previous experiments. After the 27th of May, they began 

 to die, and by the 30th none were alive. 



7. — One hundred newly-hatched shad were put in 20 ounces of mixt 

 ure, 5 per cent strength (1 ounce of refuse and 19 ounces of water) at 

 2 p. m., June 2 ; 6 p. m., 12 fish dead ; 6 a. m., June 3, 30 fish dead, 

 others weak ; 6 a. m., June 4, 40 fish dead, others weaker; 6 a. m., June 



5, 52 fish dead, remainder very weak and at the bottom ; 6 a. m., June 



6, all dead. 



8. — One hundred newly-hatched shad were put in 20 ounces of mixt- 

 ure, 1 per cent strength (1 part of refuse and 99 parts of water) at 2 

 p. m., June 2 ; 6 p. m., 9 fish dead ; 6 a. m., June 3, 16 fish dead, remain- 

 der all right; 6 a. in., June 4, 25 fish dead, remainder weakj 6 a. m., 

 J une 5, 31 fish dead ; 6 p. in., 55 fish dead ; 6 a. m., June 6, 73 fish dead ; 

 noon, June 6, all dead. 



9. — One hundred newly-hatched shad were put in 20 ounces of mixt- 

 ure, .5 per cent strength (£ part refuse and 99£ parts water), at 2 p. in., 

 June 2; 6 p. in., all alive and strong; 6 a. m., June 3, three dead, re- 

 mainder all right; 6 a. m., June 4, three dead, remainder well; 6 a. m., 

 June 5, fourteen dead, remainder fail ; 6 a. m., June 6, wenty-seven 

 dead, remainder much weaker and suffering; 6 p. in., June 6, all dead. 



10. — One hundred newly-hatched shad were put in 20 ounces of mixt- 

 ure, .25 per cent strength (.25 part of refuse and 99.75 parts water), at 

 2 p. m., June 2; 6 p. m., all well; 6 a. in., June 3, four fish dead, others 

 better than in No. 9; 6 a. m., June 4, four fish dead; 6 a. m., June 5, 

 sixteen fish dead; 6 a. in., June 6, twenty-three fish dead ; 6 p. m., fifty- 

 seven fish dead; 6 a. m., June 7, all dead. 



11. — One hundred newly-hatched shad were put in 20 ounces of Poto- 

 mac water at 2 p. m., June 2. But few are alive now (noon, June 8), and 

 these have but little vitality. 



