Bembo et al.: Stock discrimination among Engrauhs encrasicolus by means of DNA analysis 



39 



ND5/6 genes will require greater sample sizes ( prob- 

 ably around 40-50 fish). Alternatively, another re- 

 gion such as NDl/16s or the cytochrome oxidase (CO) 

 genes might be a source of complementary mtDNA 

 markers. In revealing spatial trends in accordance 

 with those recorded in previous allozyme and mtDNA 

 studies, our results underline the power, sensitivity, 

 and speed of this genetic approach to stock discrimina- 

 tion in such a highly mobile marine teleost. 



Acknowledgments 



We thank A. Belardinelli, A. Piersimoni, and D. Levi 

 for obtaining samples. Heather Hall and Paul Shaw 

 gave invaluable help with preliminary PCR work. 

 Mike O'Connell of the University of Wales Swansea 

 kindly donated the primers (adapted for use with At- 

 lantic salmon, Salmo salar) for this study. We are 

 also grateful to A. Carone, E. Arneri, G. Giannetti, 

 and the staff of the Marine and Fisheries Genetics 

 Laboratory for ideas and helpful discussion. This work 

 was funded by the European Community (EC XTV-1/ 

 MED/91/001).' 



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