Van Doornik et al : Transferrin polymorphism in Oncorhynchus kisutch 



567 



r 



Pacific Ocean 



49, 80 V iT 53l>yg6^6l. 62 



48 — r* *X'64 T 6A 



•128,129 



California 



A_ 



Figure 1 



Locations of samples. Numbers refer to sample names identified in Table 1. 



salmon samples from 34 locations in northern Or- 

 egon (north of Cape Perpetual, the Columbia River, 

 Washington coastal streams, and Puget Sound, Wash- 

 ington (Fig. 1). Starch gel electrophoresis was con- 

 ducted as described by Aebersold et al. (1987) and 

 Van Doornik et al. ( 1995). 



Observed genotype frequencies were compared 

 with expected Hardy- Weinberg proportions with a 

 chi-square goodness-of-fit test for the 48 samples for 

 which genotype-frequency data were available. 



Allele frequencies were compiled from other stud- 

 ies that included 30 or more fish per sample for the 

 transferrin locus to create a data set of 135 samples 

 (Table 1). The log-likelihood ratio statistic (G-test) 

 (Sokal and Rohlf, 1969) was used to compare allele 

 frequencies of these samples that were taken in dif- 

 ferent years at the same locale. In these analyses, 

 G-tests were performed for each polymorphic locus 



and the results were summed over all loci to yield an 

 overall G-value and a standardized G-value (G/df). 

 To test for significant differences between samples, 

 an analysis of variance (ANOVA) of arc sine square- 

 root transformed allele frequencies, for n—1 alleles, 

 was conducted with SYSTAT statistical software 

 (SYSTAT, 1992). Genetic diversity was partitioned 

 into components defined by regions, samples, and 

 within-sample (temporal variation) according to the 

 method described by Chakraborty (1980) by using 

 the BIOSYS-1 computer program (Swofford and 

 Selander, 1981). 



Genetic similarities among samples were esti- 

 mated with Nei's (1978) unbiased genetic identity 

 and projected as a dendrogram with the unweighted 

 pair-group method with arithmetic averaging 

 (UPGMA) (Sneath and Sokal, 1973) (with BIOSYS- 

 1). Genetic similarities were also analyzed by using 



