694 



Fishery Bulletin 94(4), 1 996 



PEP2*. Multiple loci encoding the same enzyme were 

 designated by consecutive numbers, with T denot- 

 ing the fastest migrating system. Alleles within each 

 locus were identified by the anodal electrophoretic 

 mobility (rounded to nearest 5%, except for FH*113) 

 of their product relative to that of the most common 

 allele observed in the spiky oreo, N. rhomboidalis, 

 which was designated '100' (cathodal migration be- 

 ing designated negative). In addition, muscle protein 

 patterns were examined after Coomassie Blue stain- 

 ing. These results are not included in the phyloge- 

 netic analyses because of uncertain homologies be- 



tween species, but patterns were species-specific. The 

 CKA* product was one of several protein products 

 visualized by this general protein stain. The mean 

 sample sizes per locus for the seven oreo species had 

 a wide range (from 14 to 598, Table 4) because poly- 

 morphic loci for the four main commercial species 

 (A. niger, A. verrucosus, N. rhomboidalis, and P. 

 maculatus) were examined in large numbers for stock 

 delineation studies (unpubl. data). 



Species relationships were analyzed with BIOSYS-1 

 software (Swofford and Selander, 1981) and PAUP 

 ( phylogenetic analysis using parsimony) 3.0s soft- 



