NOTE Purcell et al.: Genetic identification of salmonid prey from scat of Phoca vitulina nchardsi 



217 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



Chinook A 



Chinook B 



Coho 



Sockeye 



Chum 



Pink 



Steelhead 



Cutthroat 



20 DpnII 40 60 



* * * * 



TTACAATCGCTGACGGCGTGTACGGCTCTACTTTCTTTGTCGCCACCGGATTCCATGGCC 



. . . . A. 

 .T. .A. 

 .T. .A. 



DpnII 80 



100 



Apol/ Sau96I 



TACACGTGATTATTGGCTCAACCTTTCTAGCCGTTTGCCTTCTGCGACAGGTCCAATACC 

 A 



. . . . A. 

 . . . . A. 

 .T. .A. 



:. .G. 

 . . .G. 

 .T.G. 



.A. .T. 

 . AA . T . 

 . AA.T. 

 . AA.T. 



Fokl 140 160 180 



********** 



ACTTTACATCCGAACATCATTTTGGCTTTGAAGCTGCTGCTTGATATTGACACTTTGTAG 



.T. . . . 

 .T. . . . 

 .T. .G. 



200 220 start tRNA glycine 



--> 



ACGTTGTGTGACTCTTCCTATACGTCTCTATTTACTGATGAGGCTCATAATCTTTCTAGT 



.A. .G. 

 . A. . . . 



Asel 



****** 



260 



BSTNI 



280 



Start ND3 

 — > 



ATTAACACGTATAAGTGACTTCCAATCACCCGGTCTTGGTTAAAATCCAAGGAAAGATAA 



. .G 



. TGA 



. TTA 



.TTA. . .CG. 

 .T 



Apol DpnII 340 360 



****** **** 



TGAACTTAATTACAACAATCATCACTATTACCATCACATTRTCCGCAGTACTAGCCACTA 



.CG. 

 .C.G. 



.CG. 

 . . .A. 

 . . .G. 



TTTCTTTC 



Figure 1 



Aligned sequences of the 3' region of the cytochrome oxidase III gene (COM I, the tRNA glycine gene, 

 and the 5' region of the ND3 gene for seven species of the genus Oncorhynchus. The cutthroat trout 

 sequence is represented by the coastal cutthroat subspecies (O. clarki clarki). Chinook "A" refers to 

 the "A" Dpn II haplotype; chinook "B" refers to the "B" Dpn II haplotype. Sequence identity relative 

 to the chinook salmon "A" sequence is denoted by dots; nucleotide substitutions are indicated. The 

 arrow at basepair (bp) 230 is the start of the tRNA glycine gene and the arrow at bp 300 is the start 

 of the ND3 gene. Stars above the sequence correspond to restriction enzyme cut sites used in this 

 study. At position 341 in chinook. the R represents an A or G. 



