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Fishery Bulletin 102(3) 



version 4, Sunderland, MA) and pairwise comparisons 

 of sequence divergence (i.e., the number of nucleotide 

 differences between two individuals expressed as a per- 

 centage). A secondary data set, which included an ad- 



ditional 450 base pairs of control region sequence, was 

 generated for the four undetermined specimens and for 

 a subgroup of known reference species with low levels 

 of sequence divergence from the four putative pygmy 

 rockfish specimens ( Puget Sound rockfish [S. empha- 

 eus], redstripe rockfish [S. proriger], harlequin rockfish 

 IS. variegatus]. sharpchin rockfish [S. zaeentrus], and 

 pygmy rockfish). Species identifications, based on this 

 extended (cyt-fe + CR) data subset, followed analyses 

 described above. 



Results 



General development 



All 122 fish had completed notochord flexion and pos- 

 sessed a full complement of segmented fin rays by 8.1 

 mm. The mode for dorsal-fin ray counts was 13, for 

 anal-fin rays 6, and for pectoral-fin rays 17 (Table 1). 

 The mode for gill raker counts was 38, and the range 

 was 36-42. Anal- and dorsal-fin spines began to develop 

 between 9.1 and 14.0 mm. Lateral line pores began to 

 develop at 29 mm, although a full complement (37 to 46 

 pores) was not reached in our specimens. Morphometric 

 measurements were taken from 16 individual pygmy 

 rockfish of 8.1-29.6 mm (Table 2). 



Head spination 



At 8.1 mm, the postocular, parietal, nuchal, inferior post- 

 temporal, supracleithral, superior opercular, preopercu- 

 lars (with the exception of the 2 nd anterior), and 1 st and 



