Table 3 



Identification and Concentration of Major Components in the Water Soluble 



Fractions of Four Fuel Oils. 



* All values are in mg of organic per It. of sea water. 



tion from New Jersey fuel oil was lethal to the two green algae, with 

 lesser effects on the two blue-greens. The two estuarine diatoms used 

 as test organisms were not greatly inhibited by Baytown, Montana, 

 or New Jersey fuel oil water soluble extracts. However, earlier work 

 with an American Petroleum Institute fuel oil and the diatom. Thalas- 

 siosira pseudonana (3H) showed that 3H was a very sensitive organ- 

 ism. Water solubles from the Baton Rouge fuel oil were almost without 

 effect on the growth of all six microalgae. On the basis of the work 

 herein and earlier work a very cautious viewpoint is advisable in 

 generalizing on the toxicity or lack thereof of a given fuel oil on the 

 growth of different kinds of microalgae. On the other hand water 

 solubles from toxic fuel oils such as Baytown or New Jersey the data 

 clearly suggest that their potential for environmental damage is high 

 either through selective or enrichment effects on natural populations 

 or through a lowering of total primary production. 



At Florida State University, the effects on growth rates of marine 

 bacteria. Serratia marinorubra and Vibrio parahaemolyticus have 

 been determined for a variety of aromatic hydrocarbons. In general, 

 the least soluble hydrocarbons have the greatest effect on a per mole- 

 cule basis. For example, benzopyrene at a concentration of 1 .2 ppb dis- 

 played toxicity similar to naphthalene at 5.9 ppm. (Both of the above 

 concentrations represent 20% of saturation in sea water at 35%o salin- 



18 



