Table 2 



Doubling Times (Hours) of Various Microalgae Grown in Sea Water Equilibrated 

 With No 2 Fuel Oil. Numbers in Parentheses are Lag Times in Hours. 



'^V'R.-Q ^ Agmenellum quadruplicatum (blue-green), medium ASP-2 -j- B^, (6), 



temp. 39°, this laboratory. 

 MAC = Nostoc sp. (blue-green), medium CGIO (7), temp. 39°, source (D.S. Hoare, 



U.T. Austin). 

 3H ^ Thalassiosira pseudonana (diatom), medium ASP-2 + B^^ + B^ + Na2Si03. 



5B..p, temp. 30°, source (R. Guillard, Woods Hole). 

 Dun = Dunaliella tertiolecta (green), medium ASP-2 + Bj2 ~l~ ^i' temp. 32°, 



source (R. Guillard, Woods Hole). 

 Ind. 580 = Chlorella autotrophica (green), medium ASP-2 + B^^ + Bj, temp. 30°, 



source (R. Guillard, Woods Hole). 

 OBB = Gymnodinium halli (dinoflagellate), modified medium NH15 (8), temp. 



30°, source (B. Wilson, Texas A&M, Galveston). 

 Forty ml of #2 Fuel Oil layered on 320 ml filtered (Gelman type A) off-shore sea 

 water, the whole stirred gently for 24 hours at room temperature, sea water layer 

 removed and refiltered through 0.45 fiva Millipore filter. This sea water equilibrated 

 with #2 fuel oil has 15 mg total extractables/liter. Note that 50% designates 1 part 

 of algal media plus 1 part sea water saturated with API #2 fuel oil, etc. 

 3 N.D. = Not determined. 



(Table 3). Of these classes of compounds methyl, dimethyl, and tri- 

 methyl derivatives are present in relatively high concentration. 



Data indicated that anilines and phenols were extracted from oil 

 into sea water at a faster rate than naphthalenes or indoles. 



The water solubles from the four fuel oils showed considerably dif- 

 ferent inhibitory effects to growth of six microalgae, two blue-greens, 

 two greens, and two diatoms. Two of the fuel oil extracts, Baytown and 

 Montana, were lethal for blue-green algae. This was in part traceable 

 to their content of p-toluidine which was found to be toxic to Agmenel- 

 lum quadruplicatum. Strain PR-6, at a level of 1 ug in the algal lawn- 

 pad assay and 100 ug/liter in liquid culture. The water soluble frac- 



17 



